Detailed information of TA system TA197448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1571272..1571758
• Replicon: chromosome
• Accession: NZ_CP072167
• Organism: Agrobacterium larrymoorei strain AF3.44

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A4D7DPW2
• Locus tag: J5285_RS07300
• Protein ID: WP_027673882.1
• Coordinates: 1571272..1571541 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: A0A4D7E236
• Locus tag: J5285_RS07305
• Protein ID: WP_027673883.1
• Coordinates: 1571525..1571758 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5285_RS07265 (J5285_07270)	1566538..1567167	+	630	WP_027673875.1	DNA-3-methyladenine glycosylase I	-
J5285_RS07270 (J5285_07275)	1567164..1567607	+	444	WP_234881756.1	hypothetical protein	-
J5285_RS07275 (J5285_07280)	1567604..1568641	-	1038	WP_027673877.1	putative sulfate exporter family transporter	-
J5285_RS07280 (J5285_07285)	1568723..1569637	+	915	WP_027673878.1	LysR substrate-binding domain-containing protein	-
J5285_RS07285 (J5285_07290)	1569686..1570051	-	366	WP_027673879.1	DUF305 domain-containing protein	-
J5285_RS07290 (J5285_07295)	1570103..1570261	-	159	WP_157835785.1	hypothetical protein	-
J5285_RS07295 (J5285_07300)	1570504..1571268	-	765	WP_027673881.1	hypothetical protein	-
J5285_RS07300 (J5285_07305)	1571272..1571541	-	270	WP_027673882.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J5285_RS07305 (J5285_07310)	1571525..1571758	-	234	WP_027673883.1	DUF6290 family protein	Antitoxin
J5285_RS07310 (J5285_07315)	1571912..1573555	+	1644	WP_051441151.1	histidine--tRNA ligase	-
J5285_RS07315 (J5285_07320)	1573564..1574685	+	1122	WP_027673884.1	ATP phosphoribosyltransferase regulatory subunit	-
J5285_RS07320 (J5285_07325)	1574682..1575380	+	699	WP_027673885.1	ATP phosphoribosyltransferase	-
J5285_RS07325 (J5285_07330)	1575526..1575936	+	411	WP_027673886.1	GFA family protein	-
J5285_RS07330 (J5285_07335)	1575988..1576434	-	447	WP_027673887.1	DoxX family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Protein of unknown function DUF6290 (6-71)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10564.19 Da
• Isoelectric Point: 10.5156

>T197448 WP_027673882.1 NZ_CP072167:c1571541-1571272 [Agrobacterium larrymoorei]
MIWEIEFRESARKQLKKLGRQDATRIIAFLSDRISADTNPRRTGQALQGSELGKFWRYRVGDYRIICDIQDQKLVIIAIE
IGHRREVYR

Nucleotide

• Length: 270 bp

>T197448 NZ_CP096187:525599-525883 [Enterobacter roggenkampii]
ATGACTTATGAACTGGAATTTGACCCGAGGGCGTTAAAGGAGTGGAGCAAGCTGGGGGATACGGTCAAAAACCAGTTTAA
GAAGAAACTTGCCGGGGTATTGGCAAATCCCCGGGTCGAATCCGCCCGCCTGCATGGGCTGCCGGATTGCTACAAAATCA
AACTCAAGGCGTCCGGCTATCGCCTGATTTATCAGGTTCAGGACAGCGTTATAACGGTATTGGTTATCGCCGTTGGGAAA
AGAGAGAAATCAGCCGCTTATCACGACGCAAATAAACGGCTTTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 9034.08 Da
• Isoelectric Point: 4.7119

>AT197448 WP_027673883.1 NZ_CP072167:c1571758-1571525 [Agrobacterium larrymoorei]
MSKQTAIRLPDDTYERLKALSERTGRTSAFYIREAIEKHIEDMEDLYLAEEATRRHIANGERTYSLEEVMRDIDLGN

Nucleotide

• Length: 234 bp

>AT197448 NZ_CP096187:525367-525609 [Enterobacter roggenkampii]
ATGGCCACGCTAAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTATGCCGTGCTGGAAAAACTCAATATTACCCC
AACGGAAGCGGTGCGATTGCTGTTTCAGTACGTTGCTGAAACGGGGCGCATGCCGGTGAAAACGGTCACCATCAGCGACA
GCGAAGATGCGCTGATACAGACAGTCCGGGAGCGACTGGCTAATCCGCAAAAGGGAATAAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: A0A4D7DPW2

Antitoxin

• AlphaFold DB: A0A4D7E236