Detailed information of TA system TA197447

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 829654..830221
• Replicon: chromosome
• Accession: NZ_CP072167
• Organism: Agrobacterium larrymoorei strain AF3.44

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: J5285_RS03880
• Protein ID: WP_084631408.1
• Coordinates: 829654..829815 (+)
• Length: 54 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A4D7DJW9
• Locus tag: J5285_RS03885
• Protein ID: WP_027673541.1
• Coordinates: 829820..830221 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5285_RS03860 (J5285_03865)	826899..827471	+	573	WP_027673546.1	CarD family transcriptional regulator	-
J5285_RS03865 (J5285_03870)	827604..828149	-	546	WP_037170647.1	transpeptidase	-
J5285_RS03870 (J5285_03875)	828293..828976	+	684	WP_027673544.1	response regulator transcription factor	-
J5285_RS03875 (J5285_03880)	829119..829574	+	456	WP_027673543.1	cyclic nucleotide-binding domain-containing protein	-
J5285_RS03880 (J5285_03885)	829654..829815	+	162	WP_084631408.1	type II toxin-antitoxin system HicA family toxin	Toxin
J5285_RS03885 (J5285_03890)	829820..830221	+	402	WP_027673541.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
J5285_RS03890 (J5285_03895)	830235..831038	-	804	WP_027673540.1	exodeoxyribonuclease III	-
J5285_RS03895 (J5285_03900)	831127..831801	-	675	WP_027673539.1	outer membrane lipoprotein carrier protein LolA	-
J5285_RS03900 (J5285_03905)	831967..834618	-	2652	WP_027673538.1	DNA translocase FtsK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (11-50)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5841.95 Da
• Isoelectric Point: 11.8222

>T197447 WP_084631408.1 NZ_CP072167:829654-829815 [Agrobacterium larrymoorei]
MKSTDIIAALRKGSHVQFKHASKPGRVTVPHPKRDLPIGTLKSIEKQAGLKLR

Nucleotide

• Length: 162 bp

>T197447 NZ_CP096187:c290638-290336 [Enterobacter roggenkampii]
ATGAAGGAGATTGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAACTTAAAAGATCGACGAGCGAAGACCATTATCGC
TTCCCGCCTCTTTCGGTTGGCAAATGGCTTAGCGGGCGACGTTAAACCCGTGGGAGAAGGTATAAGTGAGCTAAGGATCC
ACTTTGGTCCGGGCTACAGGATCTATTTTAAAGACCAGGGCAATTACATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTGATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14301.06 Da
• Isoelectric Point: 4.6630

>AT197447 WP_027673541.1 NZ_CP072167:829820-830221 [Agrobacterium larrymoorei]
MRNYIGLIHKHADSDYGVSFPDFPGVVTAGTDLDDARLMAEEALSLHVEGLLEDGDAIPEPSSLDDVMKDGENKKAVAIL
VSLKSESKRAVRLNITLPEDVLRDIDAYAQAHGLTRSGFLARAAKHEISSASA

Nucleotide

• Length: 402 bp

>AT197447 NZ_CP096187:c290329-290042 [Enterobacter roggenkampii]
ATGCATAAATTAACACCTTACGATCCCGCCAACGCGTTGGTGGATGACGAGGAAATCGCCGTGTTTATCGCTGATGCACT
AGAAACAGGCGACTCGACATATATCGCTAAAGCCCTTGGTGTTGTCGCGCGAGCCAAAGGCATGTCGAACATTTCACAGC
AAACCGGTTTATCACGTGAGCAGCTTTATCGGTCATTCAGTGATAAAGGAAACCCAACGCTCAAAACCACGCTCGCGGTC
ATGAAAGCATTGGGTTTTGGGCTAACACTCAAACACGCCGGGGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A4D7DJW9