Detailed information of TA system TA197445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 328212..328797
• Replicon: chromosome
• Accession: NZ_CP072167
• Organism: Agrobacterium larrymoorei strain AF3.44

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A4D7DTW6
• Locus tag: J5285_RS01515
• Protein ID: WP_027676625.1
• Coordinates: 328212..328499 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: J5285_RS01520
• Protein ID: WP_027676624.1
• Coordinates: 328501..328797 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5285_RS01500 (J5285_01500)	323335..324801	+	1467	WP_027676628.1	xanthine dehydrogenase small subunit	-
J5285_RS01505 (J5285_01505)	324988..327327	+	2340	WP_027676627.1	xanthine dehydrogenase molybdopterin binding subunit	-
J5285_RS01510 (J5285_01510)	327337..328164	+	828	WP_027676626.1	xanthine dehydrogenase accessory protein XdhC	-
J5285_RS01515 (J5285_01515)	328212..328499	+	288	WP_027676625.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J5285_RS01520 (J5285_01520)	328501..328797	+	297	WP_027676624.1	putative addiction module antidote protein	Antitoxin
J5285_RS01525 (J5285_01525)	328812..329042	-	231	WP_027676623.1	hypothetical protein	-
J5285_RS01530 (J5285_01530)	329147..330040	+	894	WP_027676622.1	transcriptional regulator GcvA	-
J5285_RS01535 (J5285_01535)	330155..331402	+	1248	WP_027676621.1	urate hydroxylase PuuD	-
J5285_RS01540 (J5285_01540)	331399..332706	+	1308	WP_027676620.1	guanine deaminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (45-89)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11008.78 Da
• Isoelectric Point: 10.5155

>T197445 WP_027676625.1 NZ_CP072167:328212-328499 [Agrobacterium larrymoorei]
MIRIRKTNIFSDWFTSLRDKRAQARIQVRIDRLSLGLLGDVKFFDGIGEMRIDYGPGYRVYFTRRQNEIVILLCGGDKGS
QTRDIERAIKIAAEV

Nucleotide

• Length: 288 bp

>T197445 NZ_CP096187:c85237-84896 [Enterobacter roggenkampii]
ATGAAATCTCAACCTGCAACAACTTCGCGGGCGGTAAAGCCCTGCCTGTCACCCGTGGCAGTCTGGCAAATGCTGCTGAC
CCGACTGCTCGAAAAACACTATGGCCTGACGCTAAACGACACCCCGTTCAGTGATGAAACCGTAATTCAGGAACACATCA
ATGCCGGTATCACAATGGCTGATGCCGTGAATTTTCTGGTGGAGAAATACGAACTGGTCCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCCCCTTATCTCCGGGCTGTAGACATTCTGCGAGCGCGGCAGGCAACTGGTTTGTTGCGGCAAAG
CCGTAACAACGTAATGGGGTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10458.02 Da
• Isoelectric Point: 4.8146

>AT197445 WP_027676624.1 NZ_CP072167:328501-328797 [Agrobacterium larrymoorei]
MKVEVTDWDTSDYLNGNEAVIAYLEASFEDGDPKVIAMALGNIAKAKGMSQVAKQAGITREALYKSLSEKGDPKLSTLIG
VMKALGLRLSVAPEERAA

Nucleotide

• Length: 297 bp

>AT197445 NZ_CP096187:c85575-85258 [Enterobacter roggenkampii]
ATGTGTAACATCACATGGGGCCTGCAGCGGGATATCACGCCACGCCTGGGAGCCCGTCTGGTACAGGAGGGGAACCGGCT
GCATTATTTAGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACGCCGGATGCCGGAAGCTGGATGACACATTCCCGC
ACTTTATCAGCCAGATGGAATCCATGCTGACCATGGGCGAACTGAATCCGCAACACGCCCACTGCGTCACCCTGTATCAC
AACGGTTTCACCTGCGAAGCCGATACTCTTGGCAGTTGCGGCTACGTATACATCACCGTTTACCCCACTCCGCGCTAA

Structures

Toxin

• AlphaFold DB: A0A4D7DTW6