Detailed information of TA system TA197442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 585683..586347
• Replicon: chromosome
• Accession: NZ_CP072163
• Organism: Rhizobium sp. B21/90

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J5278_RS20865
• Protein ID: WP_209186127.1
• Coordinates: 585683..586111 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: J5278_RS20870
• Protein ID: WP_209186126.1
• Coordinates: 586108..586347 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5278_RS20845 (J5278_20860)	580749..582302	-	1554	WP_111221723.1	IS21 family transposase	-
J5278_RS20850 (J5278_20865)	582436..583530	-	1095	WP_257623488.1	group II intron reverse transcriptase/maturase	-
J5278_RS20855 (J5278_20870)	584300..584976	-	677	Protein_521	IS3 family transposase	-
J5278_RS20860 (J5278_20875)	585321..585611	-	291	WP_219258988.1	hypothetical protein	-
J5278_RS20865 (J5278_20880)	585683..586111	-	429	WP_209186127.1	type II toxin-antitoxin system VapC family toxin	Toxin
J5278_RS20870 (J5278_20885)	586108..586347	-	240	WP_209186126.1	Arc family DNA-binding protein	Antitoxin
J5278_RS20875 (J5278_20890)	586603..587317	-	715	Protein_525	IS6 family transposase	-
J5278_RS20880 (J5278_20895)	587357..587605	-	249	WP_142831608.1	hypothetical protein	-
J5278_RS20885 (J5278_20900)	587955..588299	+	345	WP_209207006.1	hypothetical protein	-
J5278_RS20890 (J5278_20905)	589360..589764	-	405	WP_209187025.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	570105..599265	29160
	-	inside	IScluster/Tn	-	-	577096..587317	10221

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Arc-like DNA binding domain (9-46)

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15224.51 Da
• Isoelectric Point: 5.8927

>T197442 WP_209186127.1 NZ_CP072163:c586111-585683 [Rhizobium sp. B21/90]
MILLDTNVISEPLKSQPDPRVAAWIDTQAVETLYVAAISLAELRYGIAVHPDGKKKQAVQEKLDRLMLPLFRGRIMPFDE
AAAQAYAELRAAAAKKGKAIGDSDGYIAATAKANGLMIATRDTSPFEAAGLTVINPWEASNG

Nucleotide

• Length: 429 bp

>T197442 NZ_CP096169:c4427839-4427309 [Shewanella sp. JNE2]
GTGAGTTATTCCAAAATTTTCAAAGAATTGGATAAATCACTACACGATAGAGCATCATTTGACTGTGGCGAAGCAGAGTT
AAACGATTTTATCCAAACTCAAGCAGCAAAACATATGCAAGCAGGTATTAGCCGTACAATGGTTTTACCTGCTGCTATGC
CATTGCCCAATCAAAAATATCCAATTTGCTCATTTTATACTATCGCACCAAGTTCAATTTGCCGTGATACCTTGCCGCAA
GCTATTGGCAAAAAGTTACCACGCTATCCCATTCCGGTCTTTCTGCTTGCTCAACTTGCGGTTCATAAAGAGTTTCATGG
CAGTGGGTTAGGTAAAGCTAGCTTAATTAAAGCACTAGAATATCTGTGGGAAATTAACGCTCACATGAGAGCTTATGCCA
TTGTTGTCGATTGCTTAACTGACCAAGCTGAATCATTCTACGCAAAATATGGTTTCGAGGTTCTTTGTGAAATCAACGGT
CGTGTCAGAATGTTTATTCCAATGAAAACGGTCGGTCAGTTATTCACTTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8686.90 Da
• Isoelectric Point: 7.2054

>AT197442 WP_209186126.1 NZ_CP072163:c586347-586108 [Rhizobium sp. B21/90]
MASITIRNVPDEVHRALRVRAAQHGRSAEAEIRIILEQAAKPEGRVKLGSLLTQIGQEVGGFDLTIERDRTPSPPVSFE

Nucleotide

• Length: 240 bp

>AT197442 NZ_CP096169:c4428105-4427836 [Shewanella sp. JNE2]
ATGGCGACTGCAAGACTTGATATCCGTTTGGATGAAGAAATCAAAGCTAAGGCTGAGAAAGCATCCGCTTTGCTCGGGTT
AAAAAGCTTAACTGAATACGTTGTTCGCTTAATGGACGAAGATGCAACTCAGGTGATAGCTGAGCATGAAAGCGTTACGG
TTAAAGCAGATGTATTCGACCAATTCATAATGGCTTGTGATGAGGCTAAGGTTCCGAATAAGGCATTGCGTGAAGCGGCT
GCATTTACTAAAAATGGTGAGTTTAAGTGA