Detailed information of TA system TA197441

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 264315..265806
• Replicon: chromosome
• Accession: NZ_CP072163
• Organism: Rhizobium sp. B21/90

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: J5278_RS19415
• Protein ID: WP_209186735.1
• Coordinates: 264315..265193 (-)
• Length: 293 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: J5278_RS19420
• Protein ID: WP_209186736.1
• Coordinates: 265186..265806 (-)
• Length: 207 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5278_RS19395 (J5278_19405)	259917..261131	-	1215	WP_219258769.1	IS701 family transposase	-
J5278_RS19400 (J5278_19410)	261245..262023	+	779	WP_257623478.1	IS5 family transposase	-
J5278_RS19405 (J5278_19415)	262069..263283	+	1215	WP_219258769.1	IS701 family transposase	-
J5278_RS31025	263573..263776	-	204	WP_247748920.1	EthD domain-containing protein	-
J5278_RS31030	264183..264305	-	123	WP_257623455.1	hypothetical protein	-
J5278_RS19415 (J5278_19425)	264315..265193	-	879	WP_209186735.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
J5278_RS19420 (J5278_19430)	265186..265806	-	621	WP_209186736.1	AbiEi antitoxin N-terminal domain-containing protein	Antitoxin
J5278_RS19425 (J5278_19435)	266524..267531	+	1008	WP_247749309.1	ABC transporter substrate-binding protein	-
J5278_RS19430 (J5278_19440)	267609..268562	+	954	WP_257623480.1	iron ABC transporter permease	-
J5278_RS19435 (J5278_19445)	268559..269332	+	774	WP_209186739.1	ABC transporter ATP-binding protein	-
J5278_RS19440 (J5278_19450)	269346..270152	+	807	WP_209186740.1	molybdate ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-261)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (12-92)

Sequences

Toxin

Protein

• Length: 293 a.a.
• Molecular weight: 32674.48 Da
• Isoelectric Point: 5.2868

>T197441 WP_209186735.1 NZ_CP072163:c265193-264315 [Rhizobium sp. B21/90]
MAKTPANMAKSVKDRLLNIARKESRAFDVLLVRFALERLLYRLSISAHRDRFVLKGGMLVTVWVADDNRVTRDADFLGHG
DADPARLIADFGEIMAIESDDGLAFDIDALAATTIREEMEYGGVRLRTAAYLERTRISVTIDIGFGDAMADATQRLDYPA
LLDFPAPQVRSYPPATVIAEKFQAMVALGVLNGRMKDYYDLWAIPRAIDIPADDLDAAIRVTFERRRTPIPRERPPGLSV
EMFSDEGKQRQWRAYAASLELEAVSLESIIGRVWELIGPSCARIVARNDNKT

Nucleotide

• Length: 879 bp

>T197441 NZ_CP096169:3388188-3388466 [Shewanella sp. JNE2]
ATGATAAAAAGTTTTAAGCACAAAGGTCTGGAAAAGTTTTATCGAACAGGCAGCACAGCGGGCATACAGGCGACTCATGC
CAAGCGACTTCGATTAATTCTATCTAACCTTGATCAAGCAGAAATCGCCGATGACATGGACCTTCCAGGACTGCGCTTAC
ATGAACTCTCAGGTTCAAGAAAAGGCACTTGGTCGGTCACTGTCAATGGTAATTGGCGCATTACGTTCAGATTCAACGGA
CGTGATGCGGAAATTGTTAACTACGAGGATTATCACTAA

Antitoxin

Protein

• Length: 207 a.a.
• Molecular weight: 22650.20 Da
• Isoelectric Point: 10.2501

>AT197441 WP_209186736.1 NZ_CP072163:c265806-265186 [Rhizobium sp. B21/90]
MPSAQDSQKLKLRSLLNERSMMHAHELREAGISAQTIARAVEGGEIDRISRGVYQKRGAEVEENQILAEAIMRAPKGVIA
LVSALAFHGLTDQMPRRVWIAIGTTDWAPVQSYPPLRMVRFTEPYLRQGIEHQVIAGIDVPVYSIPKTLADMFRNPKLVD
RSVAVEGLRAALQQRKATPSAIAQAAKAGGAWKVMQPYLEALTSNG

Nucleotide

• Length: 621 bp

>AT197441 NZ_CP096169:3388466-3388762 [Shewanella sp. JNE2]
ATGACTATGCATAACCCAGCTCACCCTGGTGAGATATTAAAAGAGTTGGTCATCGAGCCGTTGGAGTTGACGATTACCGA
TGTTGCAGAGCACTTAAATGTAAGCCGTAAAACTCTATCCAAGGTATTGAACGGACGAGGTGCAATAACTCCGGAAATGG
CCGTCCGCTTGGAACTGGCTTTTGCTAAGCCCTCTGCTGACCACTGGTTACGCCTGCAAAGCGCTTATGACTTGTCAGTA
CTCAGAAATCAAAAGGCAACGTTACACGTCCAACCTTATGAACTTGCTTTTGGATAA