Detailed information of TA system TA197439

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 3253401..3253953
• Replicon: chromosome
• Accession: NZ_CP072162
• Organism: Rhizobium sp. B21/90

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: J5278_RS15760
• Protein ID: WP_209182916.1
• Coordinates: 3253401..3253661 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: J5278_RS15765
• Protein ID: WP_209182915.1
• Coordinates: 3253645..3253953 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5278_RS15740 (J5278_15740)	3248931..3249632	-	702	WP_209182920.1	ATP phosphoribosyltransferase	-
J5278_RS15745 (J5278_15745)	3249629..3250750	-	1122	WP_209187335.1	ATP phosphoribosyltransferase regulatory subunit	-
J5278_RS15750 (J5278_15750)	3250782..3252332	-	1551	WP_209182918.1	histidine--tRNA ligase	-
J5278_RS15755 (J5278_15755)	3252562..3253332	+	771	WP_209182917.1	hypothetical protein	-
J5278_RS15760 (J5278_15760)	3253401..3253661	+	261	WP_209182916.1	BrnT family toxin	Toxin
J5278_RS15765 (J5278_15765)	3253645..3253953	+	309	WP_209182915.1	BrnA antitoxin family protein	Antitoxin
J5278_RS15770 (J5278_15770)	3254003..3254365	+	363	WP_209182914.1	DUF305 domain-containing protein	-
J5278_RS15775 (J5278_15775)	3254415..3255044	+	630	WP_209182913.1	phosphoribosyltransferase family protein	-
J5278_RS15780 (J5278_15780)	3255092..3256141	-	1050	WP_142824094.1	aldo/keto reductase	-
J5278_RS15785 (J5278_15785)	3256375..3257085	+	711	WP_209182912.1	HAD family hydrolase	-
J5278_RS15790 (J5278_15790)	3257099..3257569	-	471	WP_142824459.1	hypothetical protein	-
J5278_RS15795 (J5278_15795)	3257662..3258291	-	630	WP_209182910.1	DNA-3-methyladenine glycosylase I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-100)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9707.21 Da
• Isoelectric Point: 10.3839

>T197439 WP_209182916.1 NZ_CP072162:3253401-3253661 [Rhizobium sp. B21/90]
MKITWDERKRLANFDKHGLDFAELDADFFLAALLQPAKLGRFKAIGRRHDGTTAVIFAVLGREAVSVISMRPAHKAERSL
LNDKEN

Nucleotide

• Length: 261 bp

>T197439 NZ_CP096169:2976911-2977327 [Shewanella sp. JNE2]
TTGAATGACATTATTATCAACAAAATAGCAACCATCAAACGCTGTATAAAACGCATCCAACAAGTTTATGGTGATGGCAG
CATGTTCAAACAAGACTTCACCCTGCAAGATAGCGTTATCCTCAATTTACAACGCTGCTGCGAAGCCAGTATCGACATAG
CAAACCATATTAACCGCCAACAACAACTGGGTATCCCCCAAAGTAGCCGTGATAGCTTTACACTACTTTCACAAAATAAA
ATAATTAATCAGCAGCTTGCAGACAACTTAAAGAAGATGGTTGGCCTAAGAAACATCGCAGTACATGACTATCAAGAACT
CAACCTCGATATCGTTGTGCATGTTGTACAGCATCATCTTGAGGATTTTGAGCAATTTATTGAAGTGATAAAATTTTGTC
CAAGAATGAGTTATTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11769.13 Da
• Isoelectric Point: 6.4167

>AT197439 WP_209182915.1 NZ_CP072162:3253645-3253953 [Rhizobium sp. B21/90]
MTKKTEMRSDFQNGRGYSDQDWEEVSDNPEWTEEDFKNARPFAEVFPDLAESIRRARGRPAVEAPKRQISLRLDPDVIDA
FKATGKGWQSRINEALRKAAKI

Nucleotide

• Length: 309 bp

>AT197439 NZ_CP096169:2976496-2976918 [Shewanella sp. JNE2]
ATGCAACAACTAAATGAAAGCATTATCATCAACTTACTACAAGATAACATCCCGCAGTTGAGATTGATCTACCTATTTGG
CTCCTATGCACAGGGCACGCAACACCGCAACAGTGATATCGATATTGCGGTACTCGCCGATGTCACTTTAGACAATATCG
CCCGCTGGCAACTTGCCCAAAAACTCGCCTCAGCGTTAGATACTGATGTTGATCTTGTTGATTTACGCACGGCATCCACA
GTGTTATGTCAGCAGGTAGTCACCCAAGGAAAGCGACTTTGGGGAACGCGACAAGATGATGAGATTTTTGCAGTGAAAAC
CATCTCAATGTACCAACATCTACAAGCTGAAAGACAAGCAATTATTGATGATGCAACGGCCAAAACAACAGCCAACGATC
ACAGGGGACGTCACTTTGAATGA