Detailed information of TA system TA197433

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 55682..56375
• Replicon: plasmid unnamed3
• Accession: NZ_CP072161
• Organism: Rhizobium sp. B230/85

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: J5289_RS28930
• Protein ID: WP_209207616.1
• Coordinates: 55682..56059 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: J5289_RS28935
• Protein ID: WP_209207600.1
• Coordinates: 56040..56375 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5289_RS28915 (J5289_28895)	51710..52708	+	999	WP_209207597.1	plasmid partitioning protein RepB	-
J5289_RS28920 (J5289_28900)	52906..54228	+	1323	WP_209207598.1	plasmid replication protein RepC	-
J5289_RS28925 (J5289_28905)	54341..55258	-	918	WP_247750776.1	HEPN domain-containing protein	-
J5289_RS28930 (J5289_28910)	55682..56059	+	378	WP_209207616.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J5289_RS28935 (J5289_28915)	56040..56375	+	336	WP_209207600.1	helix-turn-helix transcriptional regulator	Antitoxin
J5289_RS28940 (J5289_28920)	56615..56926	+	312	WP_219276702.1	cold shock domain-containing protein	-
J5289_RS28945 (J5289_28925)	57134..58165	-	1032	WP_209207617.1	IS630 family transposase	-
J5289_RS28950 (J5289_28930)	58769..58921	-	153	WP_209207602.1	hypothetical protein	-
J5289_RS28955 (J5289_28935)	58921..59604	-	684	WP_209207603.1	hypothetical protein	-
J5289_RS28960 (J5289_28940)	59750..59992	+	243	WP_209207618.1	helix-turn-helix transcriptional regulator	-
J5289_RS28965 (J5289_28945)	60125..60484	-	360	WP_247750768.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..95290	95290
	-	flank	IS/Tn	-	-	57134..57880	746

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-97)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13849.81 Da
• Isoelectric Point: 9.8106

>T197433 WP_209207616.1 NZ_CP072161:55682-56059 [Rhizobium sp. B230/85]
MNQNERPLEWIGSSRKDLLKLPADVRKQFGYALHFAQHGGQHHAAKVLKGFGGAGVLEVVENDGGGTYRAVYTVRFEEAV
FVLHCFQKKSKSGIATPKEDLDIIRARLKVAEILAKEISNGNSHH

Nucleotide

• Length: 378 bp

>T197433 NZ_CP096168:3667-3978 [Shewanella sp. JNE7]
GTGAAAAAAACAGCAAGTAGTAAGAGAGCCAAGTTACCTCGCAACGTTGCGTATACCAAAACATTCCAGAGTTCATGGGA
GAGGTATAATAAGGCTGGTAAGCGAGATATGAATGTTGCCAGAGAAGTCATGACGATTTTATTCCTAGATAAGGAAATTC
CCCCAGAGTATTTAGATCATGAGCTTACTGGGTGGGATGGAGACAGGGAGCTTCACATCGGTGGTGACTTTCTCCTTGTT
TACAGGGTAAATGAGTCAACTAATCTTCTTACTTTTGTGGATATCGGTAGTCACAGCGAGCTTTTTGGGTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12030.00 Da
• Isoelectric Point: 6.4655

>AT197433 WP_209207600.1 NZ_CP072161:56040-56375 [Rhizobium sp. B230/85]
MATRIIDGIEVESSSGNVFADLGLADAEKLKIKSGLVIEIAKAVRRLDLTQEQAGHRMGLPQPKVSLLLRGDFANLSERK
LMECLSRLGYDIEITVKPTADPVGHLTFAVA

Nucleotide

• Length: 336 bp

>AT197433 NZ_CP096168:3390-3686 [Shewanella sp. JNE7]
ATGTATTCTGTAAATTCAGCGCTAAAAGCTACAGAAGTCCGTAGTCGAATTAGCCATGACTTGAAGATGGAAGCCTCTGA
TGTTTTGAATGGGTGTGGGCTAAATGTTAGCTCTGCTATTCGCTTATTTTTGGAACAAGTTGTTAGAGAACAACGCATTC
CATTTGAAGTAAAACGTCGAGTTCCGTCAGCAAAGATGGCTATTGCTCTTAAAGAGGCAAAGCTGATCGAAAAGCAATTT
TCATCTATGGATGAAATGATGAAGGAACTAGAGGCAGGTGAAAAAAACAGCAAGTAG