Detailed information of TA system TA197428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 3175..4666
• Replicon: chromosome
• Accession: NZ_CP072157
• Organism: Rhizobium sp. B230/85

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: J5289_RS18150
• Protein ID: WP_209207766.1
• Coordinates: 3788..4666 (+)
• Length: 293 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: J5289_RS18145
• Protein ID: WP_209207767.1
• Coordinates: 3175..3795 (+)
• Length: 207 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5289_RS18130 (J5289_18130)	141..1214	-	1074	WP_219276581.1	IS630 family transposase	-
J5289_RS18135 (J5289_18135)	1269..1622	-	354	WP_209207769.1	hypothetical protein	-
J5289_RS18140 (J5289_18140)	1619..2584	-	966	WP_209207768.1	P-loop NTPase fold protein	-
J5289_RS18145 (J5289_18145)	3175..3795	+	621	WP_209207767.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
J5289_RS18150 (J5289_18150)	3788..4666	+	879	WP_209207766.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
J5289_RS18155 (J5289_18155)	4663..4968	+	306	WP_209207765.1	hypothetical protein	-
J5289_RS18160 (J5289_18160)	5013..7256	-	2244	WP_209207764.1	AAA family ATPase	-
J5289_RS18165 (J5289_18165)	7433..8116	+	684	WP_209207763.1	hypothetical protein	-
J5289_RS18175 (J5289_18175)	9174..9521	+	348	WP_006204819.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	816..210099	209283
	-	flank	IS/Tn	-	-	141..1214	1073

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-263)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (16-88)

Sequences

Toxin

Protein

• Length: 293 a.a.
• Molecular weight: 32553.42 Da
• Isoelectric Point: 5.1186

>T197428 WP_209207766.1 NZ_CP072157:3788-4666 [Rhizobium sp. B230/85]
MAKAPANMAKSVKDRLLNIARKESRAFDVLLVRFALERLLYRLSISAHRDRFVLKGGMLVTVWVADDNRVTRDADFLGHG
DADPARLITDFGEIMAIESDDGLAFDIDALAATVIREEMEYGGVRLKTAAYLERTRIPVTIDIGFGDAMADATQRLDYPA
LLDFPAPQVRSYPPATVIAEKFQAMVALGVLNGRMKDYYDLWAIPRAIDIPAADLDAAIRATFERRRTPIPKERPPGLSV
TMFGDEGKQRQWRAYAATLELDAVSLESIIERVWELVGPSCARIIARNENEA

Nucleotide

• Length: 879 bp

>T197428 NZ_CP096166:c3020937-3020608 [Shewanella sp. JNE7]
ATGAGTTACACCCCAAAACGAGGTGACATAGTGTGGATTGATTTTGACCCATCTGCGGGACACGAGCAGGCTCATCATCG
TCCTGCGCTGGTACTTTCGCCTGAGCCGTTCAACAAGCAAATTCAACTTGCTTTAGTCGCGCCAATAACATCTAGGGTTA
GAGGTCATGGTTTCGAGGTTGCTGTTGATACCGACAAAACCAAAGGTGTTGTTTTGTGTCAACAAGTCAAGACGATTGAC
TACGCAGCAAGGGACGTCAAGTATATCGAAAAAGCTCCCGTTGCAGTGCTAAATGAAGTGCTCGCAAAAGTGCGAGTATT
GGTCAGTTAA

Antitoxin

Protein

• Length: 207 a.a.
• Molecular weight: 22744.34 Da
• Isoelectric Point: 10.1393

>AT197428 WP_209207767.1 NZ_CP072157:3175-3795 [Rhizobium sp. B230/85]
MPSAQDSQKVKLRSLLNARSMMHAYELREAGISAQTIARAVEGGEIERISRGVYQKRDAEIEENQILAEAIMRAPKGVIA
LVSALAFHELTDQMPRRVWIAIGTTDWAPVQSYPPLRMVRFTEPYLRQGIEHQVIAGIDVPVYSIPKTLADLFRNPKLVD
RSVAVEGLRAALKQRKATPSAIAQAAKAGGAWKVMQPYLEALTSNG

Nucleotide

• Length: 621 bp

>AT197428 NZ_CP096166:c3021191-3020937 [Shewanella sp. JNE7]
ATGCAGACCAGTATCAAAAAATGGGGGAACTCTGCTGGAGCAATTATCCCTGCTGCCGTGCTGAAGAAGGCAGGGTTCGG
TATGGGGGATCAAGTTGAAATAGAGGTTGTTCAGGGGCGTATTGTGATGAGCCCAGCAACCCCAATATTCACACTTGATG
AGTTGCTAAGTGCCAGTCCAAAAGGGTCTTTTGAACTTGATTCAGAAGATACTCAATGGCTGAGTTCGTCACCTGTAGGC
AAGGAAGAAATCTAA