Detailed information of TA system TA197427

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 2957740..2958217
• Replicon: chromosome
• Accession: NZ_CP072156
• Organism: Rhizobium sp. B230/85

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: J5289_RS14295
• Protein ID: WP_209184349.1
• Coordinates: 2957954..2958217 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: J5289_RS14290
• Protein ID: WP_209184348.1
• Coordinates: 2957740..2957967 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5289_RS14270 (J5289_14270)	2952974..2953882	+	909	WP_209206498.1	metallophosphoesterase	-
J5289_RS14275 (J5289_14275)	2954199..2954711	+	513	WP_209184346.1	RNA polymerase sigma factor	-
J5289_RS14280 (J5289_14280)	2954714..2955547	+	834	WP_209184592.1	anti-sigma factor	-
J5289_RS14285 (J5289_14285)	2955627..2957369	-	1743	WP_209184347.1	2-isopropylmalate synthase	-
J5289_RS14290 (J5289_14290)	2957740..2957967	-	228	WP_209184348.1	CopG family transcriptional regulator	Antitoxin
J5289_RS14295 (J5289_14295)	2957954..2958217	-	264	WP_209184349.1	BrnT family toxin	Toxin
J5289_RS14300 (J5289_14300)	2958254..2959444	-	1191	WP_209184350.1	benzoate/H(+) symporter BenE family transporter	-
J5289_RS14305 (J5289_14305)	2959679..2960263	-	585	WP_142823370.1	ATP-dependent Clp protease proteolytic subunit	-
J5289_RS14310 (J5289_14310)	2960493..2961311	+	819	WP_209184351.1	DUF2076 domain-containing protein	-
J5289_RS14315 (J5289_14315)	2961375..2961839	-	465	WP_142823372.1	preQ(1) synthase	-
J5289_RS14320 (J5289_14320)	2962048..2962728	+	681	WP_209184352.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10153.67 Da
• Isoelectric Point: 10.5475

>T197427 WP_209184349.1 NZ_CP072156:c2958217-2957954 [Rhizobium sp. B230/85]
MAFEFDPDKSRTNKIKHGIDLVEAQRLWIDRFLISFPARGGLESRFVAVGQIDGKHWSAICTRRHDNIRIISVRRSRKEE
IALYEGK

Nucleotide

• Length: 264 bp

>T197427 NZ_CP096166:3008803-3009057 [Shewanella sp. JNE7]
ATGAACAAAAAACATTTAAGAACGTTGACGGCTATCTTTGCTAGGCCTGTCAGCGGAGCTATCAAGTGGTCTGATATTGA
AGCGCTGTTTATTGCTCTAGGGGCTGACATTGAAGAGCGTGAGGGGTCTCGAATTGGTGTGGTGCTGTTTGGTGAAGTTC
AGGTATATCACCGCCCACATCCTCAGAAAGAAACTGACAAAGACGCAGTGATAAACATCAAAAAATGGTTAGAAAGGAAC
GGGGTGAAAGCATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8402.36 Da
• Isoelectric Point: 4.0813

>AT197427 WP_209184348.1 NZ_CP072156:c2957967-2957740 [Rhizobium sp. B230/85]
MKASELDEKFDNGEDILDYLDLTKATRPNMATQKVSVDFATSVVNSLDEEAARLGLTRQSLINLWIEERLSAAQD

Nucleotide

• Length: 228 bp

>AT197427 NZ_CP096166:3009054-3009395 [Shewanella sp. JNE7]
ATGAAAAATCTTATGATGATCAACGGGGTAAAAGCCTTTATTGATTATGATCCAGACGCTGAAACATTCCGTGGTGAGTT
CGTAGGCTTAAACGGCGGCGCGGATTTCTATGGTGAAAGCGTCGCTCAATTGGAAGCCGAGGGCGCGAAGTCATTAAGCG
TTTTTTTAGAGATGTGTCAGGAGCGTGGCATTGAGCCTTACAAAAACTTCTCTGGCAAATTCAACGTTCGGATCTCGCCA
GAGGTTCACGCTCGACTTAATGAAATTGCGCTATCGCAAAGCATTAGTTTGAATGCAGCGGTAGAAAATGCAGTCAATGA
CTACATTGCACATTCAGCGTAG