Detailed information of TA system TA197425

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/HTH_26(antitoxin)
• Location: 1479844..1480458
• Replicon: chromosome
• Accession: NZ_CP072156
• Organism: Rhizobium sp. B230/85

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: J5289_RS07040
• Protein ID: WP_209183151.1
• Coordinates: 1479844..1480089 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: J5289_RS07045
• Protein ID: WP_209187356.1
• Coordinates: 1480096..1480458 (+)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5289_RS07020 (J5289_07020)	1475886..1476161	+	276	WP_209183147.1	YlcI/YnfO family protein	-
J5289_RS07025 (J5289_07025)	1476158..1476457	+	300	WP_209183148.1	type II toxin-antitoxin system RelE/ParE family toxin	-
J5289_RS07030 (J5289_07030)	1477012..1478070	+	1059	WP_209183149.1	LacI family DNA-binding transcriptional regulator	-
J5289_RS07035 (J5289_07035)	1478098..1479750	-	1653	WP_209206631.1	choline dehydrogenase	-
J5289_RS07040 (J5289_07040)	1479844..1480089	+	246	WP_209183151.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
J5289_RS07045 (J5289_07045)	1480096..1480458	+	363	WP_209187356.1	helix-turn-helix transcriptional regulator	Antitoxin
J5289_RS07050 (J5289_07050)	1480470..1481933	-	1464	WP_209206629.1	betaine-aldehyde dehydrogenase	-
J5289_RS07055 (J5289_07055)	1481935..1483464	-	1530	WP_209183154.1	choline-sulfatase	-
J5289_RS07060 (J5289_07060)	1483461..1484060	-	600	WP_209183155.1	transcriptional regulator BetI	-
J5289_RS07065 (J5289_07065)	1484230..1484778	+	549	WP_209183156.1	HdeD family acid-resistance protein	-
J5289_RS07070 (J5289_07070)	1484980..1485234	+	255	WP_209183157.1	type II toxin-antitoxin system ParD family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (67-114)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9209.05 Da
• Isoelectric Point: 10.9851

>T197425 WP_209183151.1 NZ_CP072156:1479844-1480089 [Rhizobium sp. B230/85]
MKDVVVSRLADKALMRMQPKRRLAIIEKVKAYARGEIVDIKKMKGGNLYRIRVGQDRVIIDDKGRIVVVIDAGPRGSIYK
E

Nucleotide

• Length: 246 bp

>T197425 NZ_CP096166:c2010979-2010686 [Shewanella sp. JNE7]
ATGCCACAAATAATCTTCACCGCCACTGCAGTACGTGATTTAGAACGCTTACGTGAGTTTCTTCGCACTAAAAATCCCCC
TGCCGCACAACGGGCTGCCAGCGCCATAATCAATACCATTAGAAAACTGGAAAGCTATCCAGACATTGGTCGGCCTATCG
ATGACAATGACTTTAGCTTTAGGGAACTACTGATCGACTTTGGTGACACGGGCTATCTGGCAATGTACCAGTATGATGGC
GGCGAACGATTGACCGTATTGTGTATCCGCCACCAAAAAGAAGCCGGATATTGA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13066.79 Da
• Isoelectric Point: 4.3246

>AT197425 WP_209187356.1 NZ_CP072156:1480096-1480458 [Rhizobium sp. B230/85]
MGDIKRFEIDGKPYVLLSEEDYEDLVDGLRANAVLAGISAGEETWPLEIVEARANGGNAVRIFRNYRRMTVTELATAAGI
SQPYLSEIEAGKKTGSVDVLKRIANALKVDLDDLVVNVAD

Nucleotide

• Length: 363 bp

>AT197425 NZ_CP096166:c2011251-2010967 [Shewanella sp. JNE7]
ATGAGCACAATCAAACCCGTGTCGGTTAAATTAGATGCCGATATTAAAGCCAGAGTCGAGCATTTAGCCGAAACTCGCAA
ACGTTCATCACACTGGATGATGCGTGAAGCGATCCGTGAATACGTTGAGAGAGAAGAGAAACGAGAGGCGTTGCAGCAAG
AAGCGTTACGCGCATGGGAAGAACACCAGACATCAGGCTTGCATGTCACCGGTGACGAAGTGGTGAGTTGGCTGGAGTCC
TGGGGAAGTGAAAATGAAAAGGCTGCCCCTTCATGCCACAAATAA