Detailed information of TA system TA197422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 1220421..1220973
• Replicon: chromosome
• Accession: NZ_CP072156
• Organism: Rhizobium sp. B230/85

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: J5289_RS05805
• Protein ID: WP_209182916.1
• Coordinates: 1220713..1220973 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: J5289_RS05800
• Protein ID: WP_209182915.1
• Coordinates: 1220421..1220729 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5289_RS05770 (J5289_05770)	1216083..1216712	+	630	WP_209182910.1	DNA-3-methyladenine glycosylase I	-
J5289_RS05775 (J5289_05775)	1216805..1217275	+	471	WP_142824459.1	hypothetical protein	-
J5289_RS05780 (J5289_05780)	1217289..1217999	-	711	WP_209182912.1	HAD family hydrolase	-
J5289_RS05785 (J5289_05785)	1218233..1219282	+	1050	WP_142824094.1	aldo/keto reductase	-
J5289_RS05790 (J5289_05790)	1219330..1219959	-	630	WP_209182913.1	phosphoribosyltransferase family protein	-
J5289_RS05795 (J5289_05795)	1220009..1220371	-	363	WP_209182914.1	DUF305 domain-containing protein	-
J5289_RS05800 (J5289_05800)	1220421..1220729	-	309	WP_209182915.1	BrnA antitoxin family protein	Antitoxin
J5289_RS05805 (J5289_05805)	1220713..1220973	-	261	WP_209182916.1	BrnT family toxin	Toxin
J5289_RS05810 (J5289_05810)	1221042..1221812	-	771	WP_209182917.1	hypothetical protein	-
J5289_RS05815 (J5289_05815)	1222042..1223592	+	1551	WP_209182918.1	histidine--tRNA ligase	-
J5289_RS05820 (J5289_05820)	1223624..1224745	+	1122	WP_209187335.1	ATP phosphoribosyltransferase regulatory subunit	-
J5289_RS05825 (J5289_05825)	1224742..1225443	+	702	WP_209182920.1	ATP phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-100)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9707.21 Da
• Isoelectric Point: 10.3839

>T197422 WP_209182916.1 NZ_CP072156:c1220973-1220713 [Rhizobium sp. B230/85]
MKITWDERKRLANFDKHGLDFAELDADFFLAALLQPAKLGRFKAIGRRHDGTTAVIFAVLGREAVSVISMRPAHKAERSL
LNDKEN

Nucleotide

• Length: 261 bp

>T197422 NZ_CP096165:3680-3991 [Shewanella sp. JNE10-2]
GTGAAAAAAACAGCAAGTAGTAAGAGAGCCAAGTTACCTCGCAACGTTGCGTATACCAAAACATTCCAGAGTTCATGGGA
GAGGTATAATAAGGCTGGTAAGCGAGATATGAATGTTGCCAGAGAAGTCATGACGATTTTATTCCTAGATAAGGAAATTC
CCCCAGAGTATTTAGATCATGAGCTTACTGGGTGGGATGGAGACAGGGAGCTTCACATCGGTGGTGACTTTCTCCTTGTT
TACAGGGTAAATGAGTCAACTAATCTTCTTACTTTTGTGGATATCGGTAGTCACAGCGAGCTTTTTGGGTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11769.13 Da
• Isoelectric Point: 6.4167

>AT197422 WP_209182915.1 NZ_CP072156:c1220729-1220421 [Rhizobium sp. B230/85]
MTKKTEMRSDFQNGRGYSDQDWEEVSDNPEWTEEDFKNARPFAEVFPDLAESIRRARGRPAVEAPKRQISLRLDPDVIDA
FKATGKGWQSRINEALRKAAKI

Nucleotide

• Length: 309 bp

>AT197422 NZ_CP096165:3403-3699 [Shewanella sp. JNE10-2]
ATGTATTCTGTAAATTCAGCGCTAAAAGCTACAGAAGTCCGTAGTCGAATTAGCCATGACTTGAAGATGGAAGCCTCTGA
TGTTTTGAATGGGTGTGGGCTAAATGTTAGCTCTGCTATTCGCTTATTTTTGGAACAAGTTGTTAGAGAACAACGCATTC
CATTTGAAGTAAAACGTCGAGTTCCGTCAGCAAAGATGGCTATTGCTCTTAAAGAGGCAAAGCTGATCGAAAAGCAATTT
TCATCTATGGATGAAATGATGAAGGAACTAGAGGCAGGTGAAAAAAACAGCAAGTAG