Detailed information of TA system TA197421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-ChpS
• Location: 1048506..1049092
• Replicon: chromosome
• Accession: NZ_CP072156
• Organism: Rhizobium sp. B230/85

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: J5289_RS04970
• Protein ID: WP_209182789.1
• Coordinates: 1048763..1049092 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: J5289_RS04965
• Protein ID: WP_209182788.1
• Coordinates: 1048506..1048763 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5289_RS04960 (J5289_04960)	1046623..1048419	+	1797	WP_209187324.1	acyl-CoA dehydrogenase C-terminal domain-containing protein	-
J5289_RS04965 (J5289_04965)	1048506..1048763	+	258	WP_209182788.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
J5289_RS04970 (J5289_04970)	1048763..1049092	+	330	WP_209182789.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
J5289_RS04975 (J5289_04975)	1049140..1050348	+	1209	WP_209182790.1	acetyl-CoA C-acetyltransferase	-
J5289_RS04980 (J5289_04980)	1050440..1052653	+	2214	WP_209182791.1	FAD-dependent oxidoreductase	-
J5289_RS04985 (J5289_04985)	1053065..1053406	-	342	WP_209182792.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11877.67 Da
• Isoelectric Point: 9.8009

>T197421 WP_209182789.1 NZ_CP072156:1048763-1049092 [Rhizobium sp. B230/85]
VDRGDIWHVDLEPIRGREQAGARYVLVVTKKSFNAIGTPIVVPITTGGEFARFKGFAVSLSGAGTRSSGVIMCNQLRSLD
LRARGGKYVESVPDFIMSEVLARISTFFD

Nucleotide

• Length: 330 bp

>T197421 NZ_CP096164:c14266-13916 [Shewanella sp. JNE10-2]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9295.58 Da
• Isoelectric Point: 4.3184

>AT197421 WP_209182788.1 NZ_CP072156:1048506-1048763 [Rhizobium sp. B230/85]
MATSTLRNVGGSVMMTIPKAVVDELGLTANTKVEVLAEDGRVVAVPHKRPKYTLDELLAQCDLDAPFTEEDRQWLDDAPS
GREVI

Nucleotide

• Length: 258 bp

>AT197421 NZ_CP096164:c13911-13609 [Shewanella sp. JNE10-2]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA