Detailed information of TA system TA197418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1035345..1036009
• Replicon: chromosome
• Accession: NZ_CP072152
• Organism: Rhizobium sp. K15/93

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J5280_RS23415
• Protein ID: WP_209186127.1
• Coordinates: 1035581..1036009 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: J5280_RS23410
• Protein ID: WP_209186126.1
• Coordinates: 1035345..1035584 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5280_RS23370 (J5280_23360)	1030903..1031247	-	345	WP_142831609.1	hypothetical protein	-
J5280_RS23375 (J5280_23365)	1031331..1031600	+	270	WP_209187024.1	hypothetical protein	-
J5280_RS23380 (J5280_23370)	1031597..1031845	+	249	WP_142831608.1	hypothetical protein	-
J5280_RS23385 (J5280_23375)	1031885..1032229	+	345	Protein_965	IS6 family transposase	-
J5280_RS23390 (J5280_23380)	1032299..1032682	+	384	WP_209187039.1	transposase	-
J5280_RS23395 (J5280_23385)	1032679..1033023	+	345	WP_209187038.1	IS66 family insertion sequence element accessory protein TnpB	-
J5280_RS23400 (J5280_23390)	1033097..1034695	+	1599	WP_209187040.1	IS66 family transposase	-
J5280_RS23405 (J5280_23395)	1034705..1035089	+	385	Protein_969	DDE-type integrase/transposase/recombinase	-
J5280_RS23410 (J5280_23400)	1035345..1035584	+	240	WP_209186126.1	Arc family DNA-binding protein	Antitoxin
J5280_RS23415 (J5280_23405)	1035581..1036009	+	429	WP_209186127.1	type II toxin-antitoxin system VapC family toxin	Toxin
J5280_RS23420 (J5280_23410)	1036081..1036371	+	291	WP_209186128.1	hypothetical protein	-
J5280_RS31925	1036640..1036897	-	258	WP_257625630.1	alpha/beta hydrolase	-
J5280_RS23425 (J5280_23415)	1036830..1037333	-	504	WP_247749418.1	alpha/beta hydrolase	-
J5280_RS23430 (J5280_23420)	1037673..1038347	-	675	WP_224128900.1	permease	-
J5280_RS23435 (J5280_23425)	1038727..1039155	-	429	WP_209186130.1	hypothetical protein	-
J5280_RS23440 (J5280_23430)	1039378..1040205	-	828	WP_209186131.1	aldo/keto reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Arc-like DNA binding domain (9-46)

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15224.51 Da
• Isoelectric Point: 5.8927

>T197418 WP_209186127.1 NZ_CP072152:1035581-1036009 [Rhizobium sp. K15/93]
MILLDTNVISEPLKSQPDPRVAAWIDTQAVETLYVAAISLAELRYGIAVHPDGKKKQAVQEKLDRLMLPLFRGRIMPFDE
AAAQAYAELRAAAAKKGKAIGDSDGYIAATAKANGLMIATRDTSPFEAAGLTVINPWEASNG

Nucleotide

• Length: 429 bp

>T197418 NZ_CP096163:c4210458-4210129 [Shewanella sp. JNE10-2]
ATGAGTTACACCCCAAAACGAGGTGACATAGTGTGGATTGATTTTGACCCATCTGCGGGACACGAGCAGGCTCATCATCG
TCCTGCGCTGGTACTTTCGCCTGAGCCGTTCAACAAGCAAATTCAACTTGCTTTAGTCGCGCCAATAACATCTAGGGTTA
GAGGTCATGGTTTCGAGGTTGCTGTTGATACCGACAAAACCAAAGGTGTTGTTTTGTGTCAACAAGTCAAGACGATTGAC
TACGCAGCAAGGGACGTCAAGTATATCGAAAAAGCTCCCGTTGCAGTGCTAAATGAAGTGCTCGCAAAAGTGCGAGTATT
GGTCAGTTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8686.90 Da
• Isoelectric Point: 7.2054

>AT197418 WP_209186126.1 NZ_CP072152:1035345-1035584 [Rhizobium sp. K15/93]
MASITIRNVPDEVHRALRVRAAQHGRSAEAEIRIILEQAAKPEGRVKLGSLLTQIGQEVGGFDLTIERDRTPSPPVSFE

Nucleotide

• Length: 240 bp

>AT197418 NZ_CP096163:c4210712-4210458 [Shewanella sp. JNE10-2]
ATGCAGACCAGTATCAAAAAATGGGGGAACTCTGCTGGAGCAATTATCCCTGCTGCCGTGCTGAAGAAGGCAGGGTTCGG
TATGGGGGATCAAGTTGAAATAGAGGTTGTTCAGGGGCGTATTGTGATGAGCCCAGCAACCCCAATATTCACACTTGATG
AGTTGCTAAGTGCCAGTCCAAAAGGGTCTTTTGAACTTGATTCAGAAGATACTCAATGGCTGAGTTCGTCACCTGTAGGC
AAGGAAGAAATCTAA