Detailed information of TA system TA197416

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-ChpS
• Location: 2324241..2324827
• Replicon: chromosome
• Accession: NZ_CP072151
• Organism: Rhizobium sp. K15/93

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: J5280_RS11345
• Protein ID: WP_209182789.1
• Coordinates: 2324241..2324570 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: J5280_RS11350
• Protein ID: WP_209182788.1
• Coordinates: 2324570..2324827 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5280_RS11330 (J5280_11325)	2319926..2320267	+	342	WP_209182792.1	hypothetical protein	-
J5280_RS11335 (J5280_11330)	2320679..2322892	-	2214	WP_209182791.1	FAD-dependent oxidoreductase	-
J5280_RS11340 (J5280_11335)	2322985..2324193	-	1209	WP_209182790.1	acetyl-CoA C-acetyltransferase	-
J5280_RS11345 (J5280_11340)	2324241..2324570	-	330	WP_209182789.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
J5280_RS11350 (J5280_11345)	2324570..2324827	-	258	WP_209182788.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
J5280_RS11355 (J5280_11350)	2324914..2326710	-	1797	WP_209187324.1	acyl-CoA dehydrogenase C-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11877.67 Da
• Isoelectric Point: 9.8009

>T197416 WP_209182789.1 NZ_CP072151:c2324570-2324241 [Rhizobium sp. K15/93]
VDRGDIWHVDLEPIRGREQAGARYVLVVTKKSFNAIGTPIVVPITTGGEFARFKGFAVSLSGAGTRSSGVIMCNQLRSLD
LRARGGKYVESVPDFIMSEVLARISTFFD

Nucleotide

• Length: 330 bp

>T197416 NZ_CP096163:3239828-3240217 [Shewanella sp. JNE10-2]
ATGGATATCATCTGTTTTCCTTTTGAACGTGTGGTCGAAATTAATGCGTTAATCCTTTCGACAGAGCCCGGCATGAAAGG
TGCGGTTGATATTCCTAAACTTCAGGGAGCGTTGAGCCGTATTGACAATGCGATTGTCTACCAAGGCTTAGATGATGTGT
TTGAGATTGCTGCAAAATACACTGCTTGTATAGCGGTATCTCACGCTTGTCCTGATGCAAATAAGCGAACTGGTTTAGCA
GTAGCACTCGAATACTTATCTCTAAACGACTATGAAATCACAGAGGATAATGAACTGCTAGCGAATGCTATCCGAGACTT
AGTGCTTCAAGAGATTACCGAGACTGACTTTGCTGACATTCTTTATGCTCAGTATCTCAAGGGGTTGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9295.58 Da
• Isoelectric Point: 4.3184

>AT197416 WP_209182788.1 NZ_CP072151:c2324827-2324570 [Rhizobium sp. K15/93]
MATSTLRNVGGSVMMTIPKAVVDELGLTANTKVEVLAEDGRVVAVPHKRPKYTLDELLAQCDLDAPFTEEDRQWLDDAPS
GREVI

Nucleotide

• Length: 258 bp

>AT197416 NZ_CP096163:3239658-3239828 [Shewanella sp. JNE10-2]
ATGAATCGAAAATTAGAAGTTTACGGTGTTCAAGTGGTAACTAGACCGAAGATCAAGGCGACCAAACAACTTGATTTATC
AGGTGACTTTGGACGTCAAATCGTTAAGTCAGAAACCAAACTAGCATTAAGAACCCATCCCAAAACTTTCCAAAAGTTGG
CTGATATGTAA