Detailed information of TA system TA197415

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 2152352..2152904
• Replicon: chromosome
• Accession: NZ_CP072151
• Organism: Rhizobium sp. K15/93

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: J5280_RS10515
• Protein ID: WP_209182916.1
• Coordinates: 2152352..2152612 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: J5280_RS10520
• Protein ID: WP_209182915.1
• Coordinates: 2152596..2152904 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5280_RS10495 (J5280_10490)	2147882..2148583	-	702	WP_209182920.1	ATP phosphoribosyltransferase	-
J5280_RS10500 (J5280_10495)	2148580..2149701	-	1122	WP_209182919.1	ATP phosphoribosyltransferase regulatory subunit	-
J5280_RS10505 (J5280_10500)	2149733..2151283	-	1551	WP_209182918.1	histidine--tRNA ligase	-
J5280_RS10510 (J5280_10505)	2151513..2152283	+	771	WP_209182917.1	hypothetical protein	-
J5280_RS10515 (J5280_10510)	2152352..2152612	+	261	WP_209182916.1	BrnT family toxin	Toxin
J5280_RS10520 (J5280_10515)	2152596..2152904	+	309	WP_209182915.1	BrnA antitoxin family protein	Antitoxin
J5280_RS10525 (J5280_10520)	2152954..2153316	+	363	WP_209182914.1	DUF305 domain-containing protein	-
J5280_RS10530 (J5280_10525)	2153366..2153995	+	630	WP_209182913.1	phosphoribosyltransferase family protein	-
J5280_RS10535 (J5280_10530)	2154043..2155092	-	1050	WP_142824094.1	aldo/keto reductase	-
J5280_RS10540 (J5280_10535)	2155326..2156036	+	711	WP_209182912.1	HAD family hydrolase	-
J5280_RS10545 (J5280_10540)	2156050..2156520	-	471	WP_142824459.1	hypothetical protein	-
J5280_RS10550 (J5280_10545)	2156613..2157242	-	630	WP_209182910.1	DNA-3-methyladenine glycosylase I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-100)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9707.21 Da
• Isoelectric Point: 10.3839

>T197415 WP_209182916.1 NZ_CP072151:2152352-2152612 [Rhizobium sp. K15/93]
MKITWDERKRLANFDKHGLDFAELDADFFLAALLQPAKLGRFKAIGRRHDGTTAVIFAVLGREAVSVISMRPAHKAERSL
LNDKEN

Nucleotide

• Length: 261 bp

>T197415 NZ_CP096163:c3200508-3200215 [Shewanella sp. JNE10-2]
ATGCCACAAATAATCTTCACCGCCACTGCAGTACGTGATTTAGAACGCTTACGTGAGTTTCTTCGCACTAAAAATCCCCC
TGCCGCACAACGGGCTGCCAGCGCCATAATCAATACCATTAGAAAACTGGAAAGCTATCCAGACATTGGTCGGCCTATCG
ATGACAATGACTTTAGCTTTAGGGAACTACTGATCGACTTTGGTGACACGGGCTATCTGGCAATGTACCAGTATGATGGC
GGCGAACGATTGACCGTATTGTGTATCCGCCACCAAAAAGAAGCCGGATATTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11769.13 Da
• Isoelectric Point: 6.4167

>AT197415 WP_209182915.1 NZ_CP072151:2152596-2152904 [Rhizobium sp. K15/93]
MTKKTEMRSDFQNGRGYSDQDWEEVSDNPEWTEEDFKNARPFAEVFPDLAESIRRARGRPAVEAPKRQISLRLDPDVIDA
FKATGKGWQSRINEALRKAAKI

Nucleotide

• Length: 309 bp

>AT197415 NZ_CP096163:c3200780-3200496 [Shewanella sp. JNE10-2]
ATGAGCACAATCAAACCCGTGTCGGTTAAATTAGATGCCGATATTAAAGCCAGAGTCGAGCATTTAGCCGAAACTCGCAA
ACGTTCATCACACTGGATGATGCGTGAAGCGATCCGTGAATACGTTGAGAGAGAAGAGAAACGAGAGGCGTTGCAGCAAG
AAGCGTTACGCGCATGGGAAGAACACCAGACATCAGGCTTGCATGTCACCGGTGACGAAGTGGTGAGTTGGCTGGAGTCC
TGGGGAAGTGAAAATGAAAAGGCTGCCCCTTCATGCCACAAATAA