Detailed information of TA system TA197406

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 607833..609322
• Replicon: chromosome
• Accession: NZ_CP072147
• Organism: Rhizobium sp. K1/93

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: -
• Locus tag: J5287_RS21440
• Protein ID: WP_209186796.1
• Coordinates: 608060..609322 (+)
• Length: 421 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: J5287_RS21435
• Protein ID: WP_209186795.1
• Coordinates: 607833..608063 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5287_RS21400 (J5287_21385)	603136..603384	-	249	WP_209186791.1	hypothetical protein	-
J5287_RS21405 (J5287_21390)	603415..603762	-	348	WP_247749904.1	hypothetical protein	-
J5287_RS21410 (J5287_21395)	604051..604539	-	489	WP_209186792.1	nuclear transport factor 2 family protein	-
J5287_RS31830	604936..605155	+	220	Protein_562	IS66 family transposase	-
J5287_RS21420 (J5287_21405)	605181..605408	-	228	WP_209186807.1	DUF6429 family protein	-
J5287_RS21425 (J5287_21410)	605612..607243	-	1632	WP_209186808.1	FAD-dependent oxidoreductase	-
J5287_RS21430 (J5287_21415)	607411..607656	-	246	WP_209186794.1	hypothetical protein	-
J5287_RS21435 (J5287_21420)	607833..608063	+	231	WP_209186795.1	helix-turn-helix transcriptional regulator	Antitoxin
J5287_RS21440 (J5287_21425)	608060..609322	+	1263	WP_209186796.1	type II toxin-antitoxin system HipA family toxin	Toxin
J5287_RS21445 (J5287_21430)	609932..610211	+	280	Protein_568	helix-turn-helix domain-containing protein	-
J5287_RS21450 (J5287_21435)	610606..611415	-	810	WP_209186797.1	mechanosensitive ion channel	-
J5287_RS21455 (J5287_21440)	611646..612416	-	771	WP_209186798.1	SDR family NAD(P)-dependent oxidoreductase	-
J5287_RS21460 (J5287_21445)	613021..613449	+	429	WP_209186809.1	MucR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (151-381)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 421 a.a.
• Molecular weight: 45307.99 Da
• Isoelectric Point: 5.7044

>T197406 WP_209186796.1 NZ_CP072147:608060-609322 [Rhizobium sp. K1/93]
MTVRVLNVWWNGRIVGQFTQDQHGDIGFAYAEAWLEDEKALPLSVSLPKRPERFSRRECRPFFGGLLPEESQRLVAAQAL
GVSPANDFALLDRLGGDVAGALQLLPEDQEPIGGGLIADRQTAALDEVGIVRILDALPTRPLLAGQEGLRLSLAGAQSKA
PVVLIDGQVALPLPGQATTHILKPPIARFPGTTENEAFAMRLAAAIGLDVAPVDPRSANGRPFLLVNRYDRHHDGDGVVL
RIHQEDFCQALGVPPETKYASEGGPTFKDCFELLRRVSARPGTDIAKLLDAAIFNLVIGNADAHGKNFSILYDHSGPRMA
PLYDLLSTVAYPDLSPKLAMRIGKRATLADMDADGWQAFSKETGVSLPLVRRRVKELTDATGDAIPRLLEDMSPLAADPV
SLNRVAALIADRAKLVALSI

Nucleotide

• Length: 1263 bp

>T197406 NZ_CP096142:3085403-3085555 [Xanthomonas prunicola]
ATGAAGCGACTGCTGACACTGATGGTACTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACATGCAGGGCGCAGGTGGGAAGGTCGAGAAAACCGCCGAAAAATGCAGCGACGGTAAGTGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8069.42 Da
• Isoelectric Point: 10.5998

>AT197406 WP_209186795.1 NZ_CP072147:607833-608063 [Rhizobium sp. K1/93]
MAKQVSSPAEIGALIRSTRKAQNLRQDELAGVSGVGLRFIVDLEAGKPTAQIGKVLQVLQTLGCSIDILAPGERRK

Nucleotide

• Length: 231 bp

>AT197406 NZ_CP096142:3085630-3085764 [Xanthomonas prunicola]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTGTCGATGTTGTCGGTCGGTATGCTGTCGGGCTGCAACACCGTCGCAGGTGC
TGGCAAGGACGTGAAGGGTGCTGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA