Detailed information of TA system TA197397

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 224160..224824
• Replicon: plasmid unnamed1
• Accession: NZ_CP072143
• Organism: Rhizobium sp. L51/94

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J5274_RS25170
• Protein ID: WP_209186127.1
• Coordinates: 224396..224824 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: J5274_RS25165
• Protein ID: WP_209186126.1
• Coordinates: 224160..224399 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5274_RS25150 (J5274_25155)	220816..221061	-	246	WP_142832030.1	hypothetical protein	-
J5274_RS29145	222222..222619	-	398	Protein_205	integrase	-
J5274_RS25160 (J5274_25165)	223142..223810	-	669	WP_219275717.1	HAD family phosphatase	-
J5274_RS25165 (J5274_25170)	224160..224399	+	240	WP_209186126.1	Arc family DNA-binding protein	Antitoxin
J5274_RS25170 (J5274_25175)	224396..224824	+	429	WP_209186127.1	type II toxin-antitoxin system VapC family toxin	Toxin
J5274_RS25175 (J5274_25180)	224896..225288	+	393	WP_219275718.1	hypothetical protein	-
J5274_RS25180 (J5274_25185)	225271..226350	-	1080	WP_111223089.1	IS630 family transposase	-
J5274_RS25185 (J5274_25190)	226503..226688	+	186	WP_219275719.1	hypothetical protein	-
J5274_RS25190 (J5274_25195)	226754..226999	+	246	WP_219275720.1	hypothetical protein	-
J5274_RS25195 (J5274_25200)	227145..227899	-	755	Protein_213	SDR family oxidoreductase	-
J5274_RS25200 (J5274_25205)	228275..228901	+	627	WP_219275721.1	recombinase family protein	-
J5274_RS25205 (J5274_25210)	228908..229366	+	459	Protein_215	DUF4158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..364788	364788
	-	flank	IS/Tn	-	-	225271..226350	1079

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Arc-like DNA binding domain (9-46)

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15224.51 Da
• Isoelectric Point: 5.8927

>T197397 WP_209186127.1 NZ_CP072143:224396-224824 [Rhizobium sp. L51/94]
MILLDTNVISEPLKSQPDPRVAAWIDTQAVETLYVAAISLAELRYGIAVHPDGKKKQAVQEKLDRLMLPLFRGRIMPFDE
AAAQAYAELRAAAAKKGKAIGDSDGYIAATAKANGLMIATRDTSPFEAAGLTVINPWEASNG

Nucleotide

• Length: 429 bp

>T197397 NZ_CP096130:57053-57397 [Leptospira interrogans]
TTGGTAAAGTATAGAAACTATACTCCTGAAAAAGGAGATATAGTGTGGCTGAACTTTACTCCTCAAGCAGGTCATGAACA
GAAAGGTCGTAGACCTGCACTTGTTTTATCGCCTAAGGAATATAATTCAAAGACAGGGTTAGCAATATTTTGTCCAATTA
CCAGTAAAATTAAAGGTTATCCATTCGAAGTGTTGATTAAATCAAAAAAAATCGATGGTGTAATTCTTTCTGATCAAGTA
AAAAACTTAGATTGGACTATCCGTGAAGCAGAATTTATTGAATCAATAAATAAAGTTTCTTTAAAAGAAGTTTTAGATAA
TATTAAATTATTAATATTAACCTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8686.90 Da
• Isoelectric Point: 7.2054

>AT197397 WP_209186126.1 NZ_CP072143:224160-224399 [Rhizobium sp. L51/94]
MASITIRNVPDEVHRALRVRAAQHGRSAEAEIRIILEQAAKPEGRVKLGSLLTQIGQEVGGFDLTIERDRTPSPPVSFE

Nucleotide

• Length: 240 bp

>AT197397 NZ_CP096130:56823-57059 [Leptospira interrogans]
ATGGAATCAATTATTCAAAAATGGGGTAATAGCTTAGGAATTAGAATTCCAAAAGCAATGGCAACGGAATTAGAGTTAAA
TGATGGAAGCCATGTTGAATTACAATATGAAGGAGATAAGATTGTAATATATCCAATGAAAAAAGCTTCATTGGAAGATA
AACTTTCTAAAATTACGAAACAAAATCTTCACTCTGAAATATCTACTGGTAATTCTATAGGCAATGAAGCTTGGTAA