Detailed information of TA system TA197396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 75906..76501
• Replicon: plasmid unnamed1
• Accession: NZ_CP072143
• Organism: Rhizobium sp. L51/94

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: J5274_RS24560
• Protein ID: WP_142832462.1
• Coordinates: 76313..76501 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J5274_RS24555
• Protein ID: WP_219275884.1
• Coordinates: 75906..76307 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5274_RS24530 (J5274_24530)	71024..71914	-	891	WP_219275878.1	hypothetical protein	-
J5274_RS24535 (J5274_24535)	71992..73053	-	1062	WP_219275879.1	sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC	-
J5274_RS24540 (J5274_24540)	73151..73984	-	834	WP_219275880.1	carbohydrate ABC transporter permease	-
J5274_RS24545 (J5274_24545)	73936..74979	+	1044	WP_219275882.1	alanine racemase	-
J5274_RS24550 (J5274_24550)	75009..75830	+	822	WP_219276026.1	transporter substrate-binding domain-containing protein	-
J5274_RS24555 (J5274_24555)	75906..76307	-	402	WP_219275884.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
J5274_RS24560 (J5274_24560)	76313..76501	-	189	WP_142832462.1	type II toxin-antitoxin system HicA family toxin	Toxin
J5274_RS24565 (J5274_24565)	76822..77088	+	267	WP_219275886.1	WGR domain-containing protein	-
J5274_RS24570 (J5274_24570)	77354..77764	+	411	WP_219275888.1	SyrB-like regulator	-
J5274_RS24575 (J5274_24575)	78193..78510	+	318	WP_219275890.1	hypothetical protein	-
J5274_RS24580 (J5274_24580)	78538..79125	+	588	WP_219275892.1	hypothetical protein	-
J5274_RS24585 (J5274_24585)	79310..79480	+	171	WP_219275894.1	hypothetical protein	-
J5274_RS29095	79844..80089	+	246	Protein_87	hypothetical protein	-
J5274_RS24590 (J5274_24590)	80337..80624	+	288	WP_219275896.1	integration host factor subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..364788	364788

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6947.19 Da
• Isoelectric Point: 11.2826

>T197396 WP_142832462.1 NZ_CP072143:c76501-76313 [Rhizobium sp. L51/94]
MKSVDIISALTNDGWFEVAKKGSHVQLKHPTKRGRVTVPHPKRDIPMGTLKSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T197396 NZ_CP096129:3263666-3264064 [Leptospira interrogans]
ATGTATCTTTTGGATACCAATATATGCATTTTTCTAATTAAAAAGAAAAATGCGACTCTGTTAGAAAATCTTAAGAAAAA
ATTAAATAAAGATCTATTTGTTTCGTCTTTAACTGTTGCGGAACTTGAGTTTGGAATTCAGAAAAGTGAATTTAAAGAAA
AGAATAAAGTTGCACTAATCGAATTTTTAACGATTTTCAATATACTTTCCTTTTCTGATAAAGATGCAGAGTCTTACGGT
ATCATCAGAGCGGATTTGGAGAGGAAAGGTAATGTAATCGGTTCAATAGACATGTTACTTGCCGCTCAAGCAATTGCTAA
TAATTATATCTTTGTTACTAATAATACAAAAGAATTTAAAAGAATTAAGGCTTTAAAAATAGAAAATTGGACACAATAG

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14239.03 Da
• Isoelectric Point: 4.5442

>AT197396 WP_219275884.1 NZ_CP072143:c76307-75906 [Rhizobium sp. L51/94]
MRNYIGLIHKEAESDYGVSFPDFPGVVTAGTDLDDARRMAKEALAFHVDGLVEDGEAIPEASSLTDIMADPHNSSGTAIL
VSLKTETKKAVRVNITIPEDVLQEVDAYAEAHGLTRSGFLVQAAKREIGKIAA

Nucleotide

• Length: 402 bp

>AT197396 NZ_CP096129:3263436-3263666 [Leptospira interrogans]
ATGCAAACAGCCAAATTATTTATAAATGGAAGAAGTCAAGCGGTCAGATTACCAAAAGAATTTCAATTTACCGGAGATGA
TGTTCTCATTCAAAAAGTCGGAGAGGCGGTAATATTGGTTCCGAAAAACAAGGCCTGGAACGTATTTTTAGAAGGATTGA
ACGGGTTTTCAGATGATTTCTTTAAGGAGGAGAGAGAACAGCCGAAATCTGATAAAAGAGAAAAGCTTTAA