Detailed information of TA system TA197395

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1126611..1127191
• Replicon: chromosome
• Accession: NZ_CP072142
• Organism: Rhizobium sp. L51/94

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J5274_RS23130
• Protein ID: WP_219275265.1
• Coordinates: 1126611..1126994 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: J5274_RS23135
• Protein ID: WP_142831764.1
• Coordinates: 1126991..1127191 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5274_RS23105 (J5274_23110)	1122070..1122654	-	585	WP_219271068.1	plasmid pRiA4b ORF-3 family protein	-
J5274_RS23110 (J5274_23115)	1122654..1124312	-	1659	WP_142832615.1	IS66 family transposase	-
J5274_RS23115 (J5274_23120)	1124390..1124737	-	348	WP_046119556.1	IS66 family insertion sequence element accessory protein TnpB	-
J5274_RS23120 (J5274_23125)	1124734..1125081	-	348	WP_111217014.1	transposase	-
J5274_RS23125 (J5274_23130)	1125576..1126151	-	576	WP_257625694.1	alpha/beta hydrolase	-
J5274_RS23130 (J5274_23135)	1126611..1126994	-	384	WP_219275265.1	type II toxin-antitoxin system VapC family toxin	Toxin
J5274_RS23135 (J5274_23140)	1126991..1127191	-	201	WP_142831764.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
J5274_RS23140 (J5274_23145)	1127353..1128549	-	1197	WP_219275266.1	ROK family transcriptional regulator	-
J5274_RS23145 (J5274_23150)	1128781..1130019	+	1239	WP_219275267.1	extracellular solute-binding protein	-
J5274_RS23150 (J5274_23155)	1130081..1130980	+	900	WP_219275268.1	sugar ABC transporter permease	-
J5274_RS23155 (J5274_23160)	1130977..1131843	+	867	WP_219275269.1	carbohydrate ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1124390..1124737	347

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14065.28 Da
• Isoelectric Point: 7.4753

>T197395 WP_219275265.1 NZ_CP072142:c1126994-1126611 [Rhizobium sp. L51/94]
VILVDTSIWIDHFRHSDADLRKVIADDRLLCHPVVIGELALGSLRDRGVVMAFFSAQREALVASHDEVMKMIDRHSIFSM
GIGYTDAHLLASTLLDRRSLLWTRDKRLAAAAQKAGASLYAPTIAPN

Nucleotide

• Length: 384 bp

>T197395 NZ_CP096129:1023543-1023884 [Leptospira interrogans]
ATGATTCGTGGTGAAATTTGGTGGGTAGATTTGGGAATTCCATTTGGAAGTGAGCCTGGATTTAAACGTCCAGTTCTTAT
AATTCAAGATGACTCTTTTAATCAAAGTAATATTAATACAATAGTTTCGATTGCAATTACATCGAATTTAAATCTATCTG
AAGCACCAGGTAATGTTTTCATTAGTAAAAAAGATTCGAGTTTATCAAAGGACTCGGTCATAAATGTCTCTCAAATTGTG
ACTTTAGATAAAGAAAGATTTCTTAACAAAGCGGGTAAACTTAAATCAAATAAATTAGGTGAAGTTGAAATTGGGCTTAA
GTTGGTTACAGGCTTAGATTGA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7125.13 Da
• Isoelectric Point: 10.4525

>AT197395 WP_142831764.1 NZ_CP072142:c1127191-1126991 [Rhizobium sp. L51/94]
MRSTINLDDNLMEQARSLTGTKETAALVRQALETLVRVESGKRLAALGGTMPDAEAAPRRRSEAAK

Nucleotide

• Length: 201 bp

>AT197395 NZ_CP096129:1023308-1023556 [Leptospira interrogans]
ATGAAGACGGCGATTTCAATTCCTGATGAGTTATTTAGGGCTGCCGAGAAAATAGCTAAAAAACTTGGGATTCCTCGAAG
CCAGTTATTTGCAAAAGCATTAGAAGAATTTATCCAATCTCATAGTAAAGAATCTGTAACTGAGAAATTAAATAAAATCT
ACAGTAATAAATCCAAAGAAACTAGAAATAATATTACTGACTTATCCGTAGAATCGCTTCGTAAGAGTTTAAAAAATGAT
TCGTGGTGA