Detailed information of TA system TA197386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 7178..7871
• Replicon: plasmid unnamed2
• Accession: NZ_CP072140
• Organism: Agrobacterium sp. S7/73

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: J5276_RS30200
• Protein ID: WP_209160465.1
• Coordinates: 7494..7871 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: J5276_RS30195
• Protein ID: WP_209160463.1
• Coordinates: 7178..7513 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5276_RS30185 (J5276_30195)	4894..5169	+	276	WP_209160499.1	mobilization protein	-
J5276_RS30190 (J5276_30200)	5162..7048	+	1887	WP_219256788.1	type IV secretion system DNA-binding domain-containing protein	-
J5276_RS30195 (J5276_30205)	7178..7513	-	336	WP_209160463.1	helix-turn-helix transcriptional regulator	Antitoxin
J5276_RS30200 (J5276_30210)	7494..7871	-	378	WP_209160465.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J5276_RS30205 (J5276_30215)	7960..8301	-	342	WP_209160507.1	chromosome partitioning protein ParB	-
J5276_RS30210 (J5276_30220)	8298..8957	-	660	WP_209160467.1	AAA family ATPase	-
J5276_RS30215 (J5276_30225)	9036..9737	-	702	WP_247750057.1	SprT-like domain-containing protein	-
J5276_RS30220 (J5276_30230)	9937..10647	-	711	WP_209160470.1	helix-turn-helix domain-containing protein	-
J5276_RS30225 (J5276_30235)	10834..11043	-	210	WP_209160472.1	ribbon-helix-helix protein, CopG family	-
J5276_RS30230 (J5276_30240)	11130..11639	-	510	WP_209160474.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..18992	18992

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-97)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13991.06 Da
• Isoelectric Point: 9.4141

>T197386 WP_209160465.1 NZ_CP072140:c7871-7494 [Agrobacterium sp. S7/73]
MKQEERPLEWIGSSYKDLMQLPADVRKSVGFALHFAQHGEQHPASKVLKGFGGAGVLEVVENDVGGTYRAVYTVRYEEAV
FVLHCFQKKSKSGIATPKEDMDVIRARLKVAEAIAKEMRDANARR

Nucleotide

• Length: 378 bp

>T197386 NZ_CP096121:c78397-78116 [Acinetobacter portensis]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAGGATGATATGCAGGCTTACTTACAAGCATG
TATTGAAGAATCTAATGGCGATGCCGCATTCATTGCAAAGGCTTTAGGTAACATTGCAAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGCTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12084.05 Da
• Isoelectric Point: 10.2633

>AT197386 WP_209160463.1 NZ_CP072140:c7513-7178 [Agrobacterium sp. S7/73]
MQTRVVNGIEVTTSSGNVFADLGLPDADKLKVKSGLMIEITKAVRRLGLTQEEAGRRMGLPQPKVSALLRGDFTNLSERK
LMDALNRLGYDIEIRVRPTADPVGHLTLAVA

Nucleotide

• Length: 336 bp

>AT197386 NZ_CP096121:c78700-78398 [Acinetobacter portensis]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTCACCAAGTTAAAAGACCAGCAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAGCCGGTCGGTGAAGGTGTTTCTGAATTACGTTTCT
TCTTTGGGCCAGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTTGTTATTCTTTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTGCAATTGGCACAAGATTTAGAGGAGGAGCTATAA