Detailed information of TA system TA197384

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 525909..526579
• Replicon: plasmid pTiS7_73
• Accession: NZ_CP072138
• Organism: Agrobacterium sp. S7/73

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J5276_RS27075
• Protein ID: WP_112651585.1
• Coordinates: 526160..526579 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7U5CZ71
• Locus tag: J5276_RS27070
• Protein ID: WP_038496682.1
• Coordinates: 525909..526163 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5276_RS27060 (J5276_27070)	523379..524386	+	1008	WP_065703158.1	sensor histidine kinase	-
J5276_RS27065 (J5276_27075)	524921..525526	-	606	WP_112651681.1	J domain-containing protein	-
J5276_RS27070 (J5276_27080)	525909..526163	+	255	WP_038496682.1	plasmid stabilization protein	Antitoxin
J5276_RS27075 (J5276_27085)	526160..526579	+	420	WP_112651585.1	type II toxin-antitoxin system VapC family toxin	Toxin
J5276_RS27080 (J5276_27090)	526719..527615	-	897	WP_112153468.1	dihydrodipicolinate synthase family protein	-
J5276_RS27085 (J5276_27095)	527648..528529	-	882	WP_003517197.1	SMP-30/gluconolactonase/LRE family protein	-
J5276_RS27090 (J5276_27100)	528586..529305	-	720	WP_003517195.1	FCD domain-containing protein	-
J5276_RS27095 (J5276_27105)	529605..531554	+	1950	WP_013637433.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..1007991	1007991

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-126)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15094.26 Da
• Isoelectric Point: 4.8723

>T197384 WP_112651585.1 NZ_CP072138:526160-526579 [Agrobacterium sp. S7/73]
MILLDTNVTSEPWKPVPDEAVIAWLDAQAVETLFISAITIAELRFEIAAMPSGRRQTILRDRIEGEVLPHFSGRILSFDL
ATSQFYSELMARARASGKAIGTADGYIAATAVANGLTISTRDTSPFEAAGVKVINPWSR

Nucleotide

• Length: 420 bp

>T197384 NZ_CP096121:c60568-60281 [Acinetobacter portensis]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9297.54 Da
• Isoelectric Point: 7.3219

>AT197384 WP_038496682.1 NZ_CP072138:525909-526163 [Agrobacterium sp. S7/73]
MPAVTIRNLSEETHRALRVRAAHHGRSTEAEIRDIIEAAVRPPERIQLGSLLAAIGRDAELSNSDVEALQKSRDKTPAKP
MTFE

Nucleotide

• Length: 255 bp

>AT197384 NZ_CP096121:c60294-59980 [Acinetobacter portensis]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U5CZ71