Detailed information of TA system TA197383

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 449669..450216
• Replicon: plasmid pTiS7_73
• Accession: NZ_CP072138
• Organism: Agrobacterium sp. S7/73

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: J5276_RS26695
• Protein ID: WP_112664746.1
• Coordinates: 449923..450216 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: F0LFW5
• Locus tag: J5276_RS26690
• Protein ID: WP_013637268.1
• Coordinates: 449669..449923 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5276_RS26665 (J5276_26675)	445468..446820	+	1353	WP_065662645.1	MATE family efflux transporter	-
J5276_RS26670 (J5276_26680)	446952..447473	+	522	WP_065662646.1	Rrf2 family transcriptional regulator	-
J5276_RS26675 (J5276_26685)	447506..447973	-	468	WP_065662647.1	aspartate 1-decarboxylase	-
J5276_RS26680 (J5276_26690)	447995..448600	-	606	WP_236759717.1	Rrf2 family transcriptional regulator	-
J5276_RS26685 (J5276_26695)	448782..449474	+	693	WP_112650650.1	4'-phosphopantetheinyl transferase superfamily protein	-
J5276_RS26690 (J5276_26700)	449669..449923	+	255	WP_013637268.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
J5276_RS26695 (J5276_26705)	449923..450216	+	294	WP_112664746.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J5276_RS26700 (J5276_26710)	450477..450884	+	408	WP_080802563.1	helix-turn-helix domain-containing protein	-
J5276_RS26705 (J5276_26715)	451001..452068	+	1068	WP_209159917.1	alkene reductase	-
J5276_RS26710 (J5276_26720)	452653..453648	-	996	WP_209159918.1	LacI family DNA-binding transcriptional regulator	-
J5276_RS26715 (J5276_26725)	453824..454777	+	954	WP_019565640.1	sugar ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..1007991	1007991

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-92)

Antitoxin

• Antitoxin ParD (6-63)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11191.83 Da
• Isoelectric Point: 5.8932

>T197383 WP_112664746.1 NZ_CP072138:449923-450216 [Agrobacterium sp. S7/73]
MPFSLSVQAEEDIISIAEEGIRIFGALVARQYHDELFALLELIATNPRMARERHEISPPVRIHPFKAHLVVYRIIEDGSV
FIIRIRHSHEDWAGDSF

Nucleotide

• Length: 294 bp

>T197383 NZ_CP096120:1220402-1220698 [Acinetobacter portensis]
ATGACTTATAAACTTTTACGACATAAGGAATTTACAGCGGAATGGGAAAAGCTGCCAAGTGTAATTCGTGAGCAGTTTAA
GAAAAAATTAGCTAAGATTATTAAACAACCACATGTTCCAAAGAGTCTGTTGAGAGGTAATCTTGCAGGTTGTTGCTATA
AGATTAAATTATTAAAAGCAGGTTTTCGTCTTGTTTATCAGGTTAGAGATGATCAGGTCATTATTTTATTGGTTACAGTT
GGAAAACGAGAAGATAATGTGGTTTATGTTGAGGCAAAAAAACGAATTGAAGAATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9410.52 Da
• Isoelectric Point: 8.5776

>AT197383 WP_013637268.1 NZ_CP072138:449669-449923 [Agrobacterium sp. S7/73]
MATMNVSLPDPMKDWVEAQAKTGRYSNASDYVRDLIRRDQARTDKIAEMQRFVDDGIRSGVGSRSTEELFALATSRADIS
RGRA

Nucleotide

• Length: 255 bp

>AT197383 NZ_CP096120:1220167-1220412 [Acinetobacter portensis]
ATGGCTACTGTTAATTTTAGGGTAGATGAAGCCCTTAAAGAAAAGTCGTATTCGGTCTTAAAAGAACAAGGTATTGCACC
TACAGAGTTCTTTACAAATATTCTTGAATATATTGCTGAAACTGGGAAACTACCTGTACAAAAAGCTTTGCTTTCAGAAG
ACGATATAGAGTTATTGGCAGTTGTTCGTAAACGTATTCATGACTCTAAAGAAATGTATGAGGAAATTTCTCTAGATGAC
TTATAA

Structures

Antitoxin

• AlphaFold DB: F0LFW5