Detailed information of TA system TA197382

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 319250..319830
• Replicon: plasmid pTiS7_73
• Accession: NZ_CP072138
• Organism: Agrobacterium sp. S7/73

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J5276_RS26090
• Protein ID: WP_112651992.1
• Coordinates: 319250..319633 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F0LFX8
• Locus tag: J5276_RS26095
• Protein ID: WP_013637280.1
• Coordinates: 319630..319830 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5276_RS26065 (J5276_26080)	315716..315985	+	270	WP_112651998.1	hypothetical protein	-
J5276_RS26070 (J5276_26085)	316000..317214	+	1215	WP_112652008.1	low temperature requirement protein A	-
J5276_RS26075 (J5276_26090)	317226..317798	+	573	WP_112651996.1	DUF924 family protein	-
J5276_RS26080 (J5276_26095)	317881..318639	+	759	WP_035201237.1	type 1 glutamine amidotransferase domain-containing protein	-
J5276_RS26085 (J5276_26100)	318732..319082	+	351	WP_112651994.1	hypothetical protein	-
J5276_RS26090 (J5276_26105)	319250..319633	-	384	WP_112651992.1	type II toxin-antitoxin system VapC family toxin	Toxin
J5276_RS26095 (J5276_26110)	319630..319830	-	201	WP_013637280.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
J5276_RS26100 (J5276_26115)	320338..320706	-	369	Protein_329	transposase	-
J5276_RS26105 (J5276_26120)	320827..321993	-	1167	WP_025591801.1	IS110 family transposase	-
J5276_RS26110 (J5276_26125)	322250..322987	-	738	WP_112651872.1	SDR family oxidoreductase	-
J5276_RS26115 (J5276_26130)	323077..323907	+	831	WP_112651874.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..1007991	1007991
	-	flank	IS/Tn	-	-	320827..321993	1166

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14230.31 Da
• Isoelectric Point: 6.6942

>T197382 WP_112651992.1 NZ_CP072138:c319633-319250 [Agrobacterium sp. S7/73]
VILVDTSIWIDHFRYDDSELRKIINDDQLLCHPFVVGELALGSLRERAAVLEFLAAQREALIATHAEVMTIIDRHSIFSM
GIGYTDAHLLTSTLLDRRSSLWTRDKRLAAAAQKVGAALYPHAHASH

Nucleotide

• Length: 384 bp

>T197382 NZ_CP096120:357370-357735 [Acinetobacter portensis]
TTGAAACACAGTCGAATTTCGATTGTGTTTCAATTGGTGATTATTCTCTTAATTATTTTCATTCTATATCAATTATTAAA
TATTCTATTTCTAATCTTTTCTATTATTCTTTTAGTTATTTCATTTTTATTCTTTAATAAAAAAGAAAAAATTGAGTATT
TAGCTTATTTAGATGATGATGTTTGGAGTCTTAAATTTAAAAATAATAAAGAAATATATAGAGTTGGTATTAAAAATATT
TTAAATCATCAATTATATGTAGTGGTTCAATTCAAGCAATCTGAGCAAAAACCAATTGTTATTTGGTTTGATCAGTTAAA
TCGTACACAGTGGAAAAACTTAAAAGTATTGTCGGTGTTAAAATAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7197.28 Da
• Isoelectric Point: 10.7407

>AT197382 WP_013637280.1 NZ_CP072138:c319830-319630 [Agrobacterium sp. S7/73]
MRSTINIDDNLMERAKSLTGTKETAALVRQALETLVRVESGKRLIALGGSMPEAEASPRRRSDVAK

Nucleotide

• Length: 201 bp

>AT197382 NZ_CP096120:357099-357359 [Acinetobacter portensis]
ATGACTGAAGAAATTAGTTTAGAAGAACGTAAAGTTATTTACCGTGCACGTCGTGGCTTAAAAGAAATTGATGTGTATTT
TGATCCGTACGTTAAAAACTACTATTTGAAAGCAGATGTTGAAGAAAAAGAGATGTTTAATGAGTTAGTCGCTCAAGAAG
ATCCTGATCTTTTAGACTGGTTTATGGAGGTTTCTGAGCCACCACGTCCAGAACTTAAAGATTTTATTAAGAAATTGAAA
TTTTATGTGCATGGTGCATAG

Structures

Antitoxin

• AlphaFold DB: F0LFX8