Detailed information of TA system TA197380

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 2296501..2296963
• Replicon: chromosome
• Accession: NZ_CP072136
• Organism: Agrobacterium sp. S7/73

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A9E8LFT7
• Locus tag: J5276_RS11410
• Protein ID: WP_065703878.1
• Coordinates: 2296712..2296963 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: F0L6V5
• Locus tag: J5276_RS11405
• Protein ID: WP_013635548.1
• Coordinates: 2296501..2296728 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5276_RS11380 (J5276_11380)	2292002..2292448	+	447	WP_004432207.1	DoxX family protein	-
J5276_RS11385 (J5276_11385)	2292513..2293205	-	693	WP_004432208.1	ATP phosphoribosyltransferase	-
J5276_RS11390 (J5276_11390)	2293202..2294329	-	1128	WP_004432209.1	ATP phosphoribosyltransferase regulatory subunit	-
J5276_RS11395 (J5276_11395)	2294346..2294714	-	369	WP_026330619.1	VOC family protein	-
J5276_RS11400 (J5276_11400)	2294719..2296344	-	1626	WP_065703880.1	histidine--tRNA ligase	-
J5276_RS11405 (J5276_11405)	2296501..2296728	+	228	WP_013635548.1	DUF6290 family protein	Antitoxin
J5276_RS11410 (J5276_11410)	2296712..2296963	+	252	WP_065703878.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J5276_RS11415 (J5276_11415)	2296970..2297734	+	765	WP_013635546.1	hypothetical protein	-
J5276_RS11420 (J5276_11420)	2297786..2298703	-	918	WP_004432220.1	LysR substrate-binding domain-containing protein	-
J5276_RS11425 (J5276_11425)	2298786..2299829	+	1044	WP_019564245.1	YeiH family protein	-
J5276_RS11430 (J5276_11430)	2299845..2300288	-	444	WP_004432223.1	hypothetical protein	-
J5276_RS11435 (J5276_11435)	2300285..2300914	-	630	WP_004432231.1	DNA-3-methyladenine glycosylase I	-
J5276_RS11440 (J5276_11440)	2301152..2301925	+	774	WP_004432233.1	L,D-transpeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (12-82)

Antitoxin

• Protein of unknown function DUF6290 (6-73)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9851.38 Da
• Isoelectric Point: 9.5687

>T197380 WP_065703878.1 NZ_CP072136:2296712-2296963 [Agrobacterium sp. S7/73]
VIWTIEYDVLVQKEMRKIDPQTRQRIRVAALDNPRQTGAALQGSELGSFWRYRVGDYRIICDIQDHKLVVLVVEIGHRRE
IYR

Nucleotide

• Length: 252 bp

>T197380 NZ_CP072136:2296712-2296963 [Agrobacterium sp. S7/73]
GTGATCTGGACAATTGAATACGATGTTCTTGTCCAGAAAGAGATGCGGAAAATCGATCCGCAGACGCGTCAGCGTATCCG
AGTAGCGGCGCTCGACAATCCCCGCCAGACAGGTGCCGCCCTGCAAGGCTCAGAACTCGGCAGCTTCTGGCGTTACCGGG
TAGGCGATTACCGTATCATCTGCGATATACAGGACCACAAGCTTGTCGTGCTGGTGGTGGAAATTGGCCATCGCCGCGAA
ATCTACCGTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8909.92 Da
• Isoelectric Point: 4.7254

>AT197380 WP_013635548.1 NZ_CP072136:2296501-2296728 [Agrobacterium sp. S7/73]
MSKQTAIRLPDETYERLKALSERTGRTSAYYIREAIEKHIEDMEDLYLAEEATRRIQRGESKVVSAEEFWRDLDN

Nucleotide

• Length: 228 bp

>AT197380 NZ_CP072136:2296501-2296728 [Agrobacterium sp. S7/73]
ATGAGCAAACAGACTGCCATCCGCCTGCCGGACGAGACCTATGAACGGCTGAAAGCGCTTTCCGAGCGCACGGGCCGTAC
GTCCGCATACTATATCCGCGAGGCGATTGAGAAACATATTGAGGATATGGAAGATCTCTATCTAGCCGAAGAGGCGACGC
GGCGCATCCAGCGCGGTGAATCAAAGGTGGTAAGCGCCGAGGAGTTCTGGCGTGATCTGGACAATTGA

Structures

Antitoxin

• AlphaFold DB: F0L6V5