Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5441653..5442278 Replicon chromosome
Accession NZ_CP072130
Organism Klebsiella variicola strain F2R9

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag J5475_RS26180 Protein ID WP_008807903.1
Coordinates 5441653..5442036 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag J5475_RS26185 Protein ID WP_004150355.1
Coordinates 5442036..5442278 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5475_RS26165 (J5475_26160) 5439019..5439921 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
J5475_RS26170 (J5475_26165) 5439918..5440553 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
J5475_RS26175 (J5475_26170) 5440550..5441479 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
J5475_RS26180 (J5475_26175) 5441653..5442036 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
J5475_RS26185 (J5475_26180) 5442036..5442278 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
J5475_RS26190 (J5475_26185) 5442483..5443400 + 918 WP_023340866.1 alpha/beta hydrolase -
J5475_RS26195 (J5475_26190) 5443415..5444356 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
J5475_RS26200 (J5475_26195) 5444401..5444838 - 438 WP_008807906.1 D-aminoacyl-tRNA deacylase -
J5475_RS26205 (J5475_26200) 5444835..5445695 - 861 WP_008807907.1 virulence factor BrkB family protein -
J5475_RS26210 (J5475_26205) 5445689..5446288 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T197379 WP_008807903.1 NZ_CP072130:c5442036-5441653 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T197379 NZ_CP096118:c4700910-4700536 [Salmonella enterica subsp. enterica serovar Indiana]
ATGGTCAAAGGGTCTGCGCTTTTTGATACCAATATCCTCATCGACCTGTTTAGCGGTCGAATTGAAGCGAAACATGCGCT
GGAAGCGTATCCGCCACAGAATGCGATTAGCCTTATCACATGGATGGAAGTGATGGTGGGTGCTAAGAAATATCATCAGG
AAAACCGCACGCGCATTGCATTAAGTGCGTTCAATATTATTGGCGTAACACAGGAAATCGCGGAGCGAAGCGTCATTGTC
AGGCAGGAGTATGGCATGAAACTCCCGGATGCGATCATTCTGGCCACTGCACAAGTCCATCGTTGTGAGCTTGTGACGCG
TAATACAAAAGACTTTGCCGATATTCCGGGAGTGATCACGCCGTATCACTTGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT197379 WP_004150355.1 NZ_CP072130:c5442278-5442036 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT197379 NZ_CP096118:c4701152-4700910 [Salmonella enterica subsp. enterica serovar Indiana]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGATGTGATTAAACGGCTTGACGATCTCAAGGT
GCAACGCAACCTACCTCGTGCAGAACTCTTGCGGGAGGCCGTTGAGCAGTATCTGGAAAGACAGGATCGGGCTGAGACCA
CAATTTCGAAAGCATTGGGGCTCTGGCAAGGCTGTGAGGAAGATGGCGTTGAATACCAACGTAAACTTCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References