Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4214009..4214628 Replicon chromosome
Accession NZ_CP072130
Organism Klebsiella variicola strain F2R9

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag J5475_RS20400 Protein ID WP_002892050.1
Coordinates 4214410..4214628 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag J5475_RS20395 Protein ID WP_008805436.1
Coordinates 4214009..4214383 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5475_RS20385 (J5475_20380) 4209164..4210357 + 1194 WP_040973691.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
J5475_RS20390 (J5475_20385) 4210380..4213526 + 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit AcrB -
J5475_RS20395 (J5475_20390) 4214009..4214383 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
J5475_RS20400 (J5475_20395) 4214410..4214628 + 219 WP_002892050.1 HHA domain-containing protein Toxin
J5475_RS20405 (J5475_20400) 4214787..4215353 + 567 WP_022066286.1 maltose O-acetyltransferase -
J5475_RS20410 (J5475_20405) 4215325..4215450 - 126 Protein_4019 hypothetical protein -
J5475_RS20415 (J5475_20410) 4215490..4215960 + 471 WP_008805434.1 YlaC family protein -
J5475_RS20420 (J5475_20415) 4215929..4217386 - 1458 WP_022066287.1 PLP-dependent aminotransferase family protein -
J5475_RS20425 (J5475_20420) 4217487..4218185 + 699 WP_022066288.1 GNAT family protein -
J5475_RS20430 (J5475_20425) 4218182..4218322 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
J5475_RS20435 (J5475_20430) 4218322..4218585 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T197377 WP_002892050.1 NZ_CP072130:4214410-4214628 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T197377 NZ_CP096118:3782839-3783090 [Salmonella enterica subsp. enterica serovar Indiana]
ATGCGGGATACTTACAAAACCCACATGACCACCTATTACTCGCGCATCCCCAGCCTGCACCTTAAGGGCGACTGGCTGGC
AGAAGCGGGATTTGATACCGGGGCGAGCGTGACCGTGAAGATCTCCGAAGGGTGTTTGACTCTGATTGTGGAGACAGACG
AAGTGAGGGATCTACGGAGGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCACATTAAGGCGGGGGTATATGATGTGGTG
AACGGGAATTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT197377 WP_008805436.1 NZ_CP072130:4214009-4214383 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT197377 NZ_CP096118:c3783080-3782855 [Salmonella enterica subsp. enterica serovar Indiana]
TTCACCACATCATATACCCCCGCCTTAATGTGCTTCATCGACTTTTTCACCTGATAAAGCTCCCTCCGTAGATCCCTCAC
TTCGTCTGTCTCCACAATCAGAGTCAAACACCCTTCGGAGATCTTCACGGTCACGCTCGCCCCGGTATCAAATCCCGCTT
CTGCCAGCCAGTCGCCCTTAAGGTGCAGGCTGGGGATGCGCGAGTAATAGGTGGTCATGTGGGTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References