Detailed information of TA system TA197365

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1646701..1647698
• Replicon: chromosome
• Accession: NZ_CP072130
• Organism: Klebsiella variicola strain F2R9

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: J5475_RS26575
• Protein ID: WP_229531768.1
• Coordinates: 1647144..1647698 (-)
• Length: 185 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: J5475_RS08065
• Protein ID: WP_040968744.1
• Coordinates: 1646701..1647147 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5475_RS08050 (J5475_08050)	1642996..1643994	+	999	WP_008804028.1	galactose/glucose ABC transporter substrate-binding protein MglB	-
J5475_RS08055 (J5475_08055)	1644113..1645633	+	1521	WP_008804029.1	galactose/methyl galactoside ABC transporter ATP-binding protein MglA	-
J5475_RS08060 (J5475_08060)	1645649..1646659	+	1011	WP_002912871.1	galactose/methyl galactoside ABC transporter permease MglC	-
J5475_RS08065 (J5475_08065)	1646701..1647147	-	447	WP_040968744.1	helix-turn-helix transcriptional regulator	Antitoxin
J5475_RS26575	1647144..1647698	-	555	WP_229531768.1	HipA domain-containing protein	Toxin
J5475_RS26580	1647727..1648248	-	522	WP_023340544.1	hypothetical protein	-
J5475_RS08075 (J5475_08075)	1648363..1649094	-	732	WP_008804033.1	outer membrane permeability protein SanA	-
J5475_RS08080 (J5475_08080)	1649387..1650271	-	885	WP_012541034.1	cytidine deaminase	-
J5475_RS08085 (J5475_08085)	1650400..1651095	-	696	WP_008804035.1	CidB/LrgB family autolysis modulator	-
J5475_RS08090 (J5475_08090)	1651085..1651489	-	405	WP_004201620.1	CidA/LrgA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (2-147)

Antitoxin

• Cro/C1-type helix-turn-helix domain (78-132)

Sequences

Toxin

Protein

• Length: 185 a.a.
• Molecular weight: 20710.03 Da
• Isoelectric Point: 5.7891

>T197365 WP_229531768.1 NZ_CP072130:c1647698-1647144 [Klebsiella variicola]
MRLGMESVYSLLQKAPATILDHETTLRILIEKITSSNMVRVQNYRFDVQGFIIDWVRRDLLNIIFGNSDNHGRNTAFMKA
DNEIILAPIYDFAPMKADPEGIPRTMKWSLACESGGEYNFGAIAQALAEWITPDTLLNALSETASQLVDLPSRLKVRGVP
VQILEMPSIGFKFIPDKLARWGLL

Nucleotide

• Length: 555 bp

>T197365 NZ_CP096114:c1925651-1925496 [Staphylococcus argenteus]
ATGTCTAAAGATAAAGATGCAAAATTAAATTATCACGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAGATGGAACAAATTTCAGATCAAAATGATCAAGAGAAGAGTACTGATGAAAAGAATAGCCATTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16422.09 Da
• Isoelectric Point: 10.4705

>AT197365 WP_040968744.1 NZ_CP072130:c1647147-1646701 [Klebsiella variicola]
MKTLNKKETEIQNTLARLRADNPPSLPATGVTATEKKARSEISRQRVSAGLKKVKTVDRQAVIHAIIYDIMLGAISQGEA
LKKLRVEVLGLRQDEYARLVDVSRKTLSDVENDKGNYSAEIINKIYKPFGLETGLVPISKTLISSLFK

Nucleotide

• Length: 447 bp

>AT197365 NZ_CP096114:c1926283-1925684 [Staphylococcus argenteus]
ATGGCAATGAACTTTAAAGTATTTGACAACAGTCAACTTGTAGCAGAATATGCTGCAGATATTATTAGGAAACAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTGGATACAGACCAAGCACCAGTATTAGATGAACTAAAGAAAAACGTTG
AGAAACACGCTGTTGATTTTAGTCAAATAAATATTTTAGATTACGACAATAAAAAATCTTATTTTGAAGCATTAGGCGTG
CCAGCAAGTCAAATTTATCCAATTGCGTTTGAAAAAGATGCTATTGAATTTATCTCTGACAAAATTAAAACTAAAGAAAA
TAAAGGCAAACTAACATTACAAGTTGTTTCTATTGATGAGCAAGGAAAATTAAATGTTAGTATCCGCCAAGGTTTAATGG
AAGCGAGAGAAATATTTTTAGTTGTGACAGGTGCTAATAAAAAGGAAGTTGTTGAAAAATTATACCAAGAAAATGGTAAA
ACTAGTTTCGAACCATCTGACTTAAAATCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTCAAAGCTTACTTTACATCTCGCTTTGCTTAA