Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 825300..825957 Replicon chromosome
Accession NZ_CP072130
Organism Klebsiella variicola strain F2R9

Toxin (Protein)


Gene name cptA Uniprot ID W8UCT0
Locus tag J5475_RS04075 Protein ID WP_002916310.1
Coordinates 825547..825957 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag J5475_RS04070 Protein ID WP_002916312.1
Coordinates 825300..825566 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5475_RS04045 (J5475_04045) 821742..822470 - 729 WP_012967245.1 MurR/RpiR family transcriptional regulator -
J5475_RS04050 (J5475_04050) 822521..822832 + 312 WP_008806430.1 N(4)-acetylcytidine aminohydrolase -
J5475_RS04055 (J5475_04055) 822996..823655 + 660 WP_008806429.1 hemolysin III family protein -
J5475_RS04060 (J5475_04060) 823686..824024 + 339 WP_136071171.1 hypothetical protein -
J5475_RS04065 (J5475_04065) 824071..825054 - 984 WP_008806428.1 tRNA-modifying protein YgfZ -
J5475_RS04070 (J5475_04070) 825300..825566 + 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
J5475_RS04075 (J5475_04075) 825547..825957 + 411 WP_002916310.1 protein YgfX Toxin
J5475_RS04080 (J5475_04080) 825964..826485 - 522 WP_008806427.1 flavodoxin FldB -
J5475_RS04085 (J5475_04085) 826586..827482 + 897 WP_008806426.1 site-specific tyrosine recombinase XerD -
J5475_RS04090 (J5475_04090) 827505..828218 + 714 WP_008806425.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
J5475_RS04095 (J5475_04095) 828224..829957 + 1734 WP_008806424.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16049.85 Da        Isoelectric Point: 11.4778

>T197364 WP_002916310.1 NZ_CP072130:825547-825957 [Klebsiella variicola]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 411 bp

>T197364 NZ_CP096114:c840886-840698 [Staphylococcus argenteus]
ATGACTTTACATTTTGCAATTTTATTTTGGCTGGCATTAATTTTTATCGTTGCTGGTACATTTATATTTGTCTTAATGAA
AAAAACTGCTAAAGAATCTAAAAAAGAATCTTATTTAAGCTTCGCTGTTATTTTCTATATTTTTGGATTCGCAATGCTAA
TTTATACATTAATATTTGGAATTCTTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT197364 WP_002916312.1 NZ_CP072130:825300-825566 [Klebsiella variicola]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT197364 NZ_CP096114:c841175-840915 [Staphylococcus argenteus]
ATGAAAAACATTCAATTAAATGCTATAAATTTAGTTATAACAATCATATTTGCTATCTTTAACATTATGATTACATATAA
CAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGCATTATCATTTGTGGTTCGATACTCATTGTATCGTTTACCATTG
CAATGATTACTAAATTCTGGTTAAGTGAAATATTATTTTTTATTAACATCGTGCTCGTTCTTTATTATATTTATCCAATT
TTTTATGATTTCATTAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GSW7

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References