Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 287521..288152 Replicon chromosome
Accession NZ_CP072130
Organism Klebsiella variicola strain F2R9

Toxin (Protein)


Gene name hicA Uniprot ID A0A2V3KJT3
Locus tag J5475_RS01305 Protein ID WP_044650325.1
Coordinates 287521..287796 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID R4YIG2
Locus tag J5475_RS01310 Protein ID WP_004181489.1
Coordinates 287793..288152 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5475_RS01285 (J5475_01285) 282757..283092 + 336 WP_002921203.1 universal stress protein UspB -
J5475_RS01290 (J5475_01290) 283152..284648 - 1497 WP_008806938.1 inorganic phosphate transporter PitA -
J5475_RS01295 (J5475_01295) 284878..286071 + 1194 WP_012967106.1 NAD(P)/FAD-dependent oxidoreductase -
J5475_RS01300 (J5475_01300) 286111..287124 - 1014 WP_012540371.1 magnesium transporter -
J5475_RS01305 (J5475_01305) 287521..287796 + 276 WP_044650325.1 type II toxin-antitoxin system HicA family toxin Toxin
J5475_RS01310 (J5475_01310) 287793..288152 + 360 WP_004181489.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
J5475_RS01315 (J5475_01315) 288180..288581 - 402 WP_032733900.1 nickel-responsive transcriptional regulator NikR -
J5475_RS01320 (J5475_01320) 288569..289360 - 792 WP_040968932.1 nickel import ATP-binding protein NikE -
J5475_RS01325 (J5475_01325) 289357..290121 - 765 WP_008806946.1 nickel import ATP-binding protein NikD -
J5475_RS01330 (J5475_01330) 290121..290954 - 834 WP_012540374.1 nickel ABC transporter permease subunit NikC -
J5475_RS01335 (J5475_01335) 290951..291895 - 945 WP_008806948.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10279.91 Da        Isoelectric Point: 10.3165

>T197361 WP_044650325.1 NZ_CP072130:287521-287796 [Klebsiella variicola]
MEQQVLSLRNKQRHTLEQLFKIPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVKP

Download         Length: 276 bp

>T197361 NZ_CP096040:c4293904-4293569 [Caulobacter segnis]
GTGAGCGCGCGTTATGTCCCCGACGCCGGCGATATTGTTTGGCTGCAATTTGATCCTCAAGCAGGCCATGAGCAGGCTGG
CCACCGTCCCGCGTTGGTGCTCTCGCCGGCCGCTTACAACGGCAAGCGCGGCATGATGTTGTGTTGTCCGATGACTAGTC
GGATCAAGGGCTACCCATTCGAAGTCGTTGTTCCAGGCGTCTCGCCCAGCGCCGTACTGGCTGATCAGATCAAAAGCGTC
GATTGGGTCGCTCGCCGCGCGGCGCGCAAGGATGTGGCGCCGCCTGGTGTCCTTGCCGAGACCCGCGCCAAGCTGAAGGC
CTTGCTAAGTGTCTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13313.02 Da        Isoelectric Point: 4.4605

>AT197361 WP_004181489.1 NZ_CP072130:287793-288152 [Klebsiella variicola]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCHEAGIEPYAHPEKMKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp

>AT197361 NZ_CP096040:c4294143-4293901 [Caulobacter segnis]
ATGCACGTCGTCGTGAAGAAGTGGGGTAACAGCGCTTCGGTCCGCATTCCGGCCCGCGTGATGGCGGCGGCGGATCTGAG
TATCGATCAGGCGGTCGACGTTCGCGAAGAGAACGGACGCATCATCATCGAGCCGATCCGGGAGCCGGAATACGATCTGG
ACGCGATGCTGGCGGAGATGGAGCCCGATACGTTTCACGAGGATGTCGACTTTGGCGCGCCTGCCGGGAAGGAAGTCTGG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V3KJT3

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2M5Q1

References