Detailed information of TA system TA197354

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 3057516..3058433
• Replicon: chromosome
• Accession: NZ_CP072120
• Organism: Bacillus amyloliquefaciens strain GKT04

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: J5X95_RS15690
• Protein ID: WP_007407256.1
• Coordinates: 3057687..3058433 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: J5X95_RS15685
• Protein ID: WP_003154807.1
• Coordinates: 3057516..3057686 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5X95_RS15650 (3052740)	3052740..3054371	+	1632	WP_052827325.1	hypothetical protein	-
J5X95_RS15655 (3054384)	3054384..3054755	+	372	WP_043021266.1	XkdW family protein	-
J5X95_RS15660 (3054760)	3054760..3054957	+	198	WP_012117366.1	XkdX family protein	-
J5X95_RS15665 (3055014)	3055014..3055775	+	762	WP_015239693.1	hypothetical protein	-
J5X95_RS15670 (3055827)	3055827..3056090	+	264	WP_003154815.1	hemolysin XhlA family protein	-
J5X95_RS15675 (3056104)	3056104..3056367	+	264	WP_003154813.1	phage holin	-
J5X95_RS15680 (3056381)	3056381..3057259	+	879	WP_020955695.1	N-acetylmuramoyl-L-alanine amidase	-
J5X95_RS15685 (3057516)	3057516..3057686	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
J5X95_RS15690 (3057687)	3057687..3058433	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
J5X95_RS15695 (3058538)	3058538..3059536	-	999	WP_007407255.1	inorganic phosphate transporter	-
J5X95_RS15700 (3059549)	3059549..3060166	-	618	WP_003154804.1	DUF47 domain-containing protein	-
J5X95_RS15705 (3060452)	3060452..3061768	-	1317	WP_007407254.1	amino acid permease	-
J5X95_RS15710 (3062093)	3062093..3063043	+	951	WP_012117369.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T197354 WP_007407256.1 NZ_CP072120:c3058433-3057687 [Bacillus amyloliquefaciens]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T197354 NZ_CP096034:241754-242104 [Gottfriedia acidiceleris]
TTGATAGTAAAACGTGGCGACGTATATTTTGCAGACCTTTCCCCTGTAGTTGGCTCAGAACAAGGTGGAGTTCGTCCGGT
TCTCATAATACAAAATGATATCGGTAATCGTTTTAGTCCTACCGTTATAGTCGCTGCGATTACAGCACAAATCCAAAAAG
CAAAGCTTCCAACACATGTTGAAATCGATGCTAAAAAATATGGATTTGAACGTGATTCGGTTATACTACTAGAACAAATT
CGAACGATTGACAAACAACGATTAACCGATAAGATAACACATCTTGATGATGAGATGATGGATCAGGTTGACGAAGCGTT
GCAAATCAGTTTAGGACTTGTTGATTTTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT197354 WP_003154807.1 NZ_CP072120:c3057686-3057516 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT197354 NZ_CP096034:241468-241749 [Gottfriedia acidiceleris]
TTGTCCGAACTAAATGCGACAACTGAAATAACAGTACGTTTACCAAAACATGTAGTAAGCGAACTAGATGGATTAGTAAA
GCAAGAAAACGGTAATCGAAATGAGTATATTTATCAAGCGACTAAGATGTTTTTAAAAGATCGTAAAAGACGCCAAAGTC
GCGAACAAATGCGACGTGGATACATGGAAATGGCTAAAATCAACTTATTAATATCTTCTGAGTCATTCTATGCTGAGTCT
GAAGCGGATTCTAAAGTGGAGCGCTTAGTTAGCGGGGGGTAA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14