Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2303044..2303681 Replicon chromosome
Accession NZ_CP072120
Organism Bacillus amyloliquefaciens strain GKT04

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag J5X95_RS11700 Protein ID WP_003156187.1
Coordinates 2303331..2303681 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag J5X95_RS11695 Protein ID WP_003156188.1
Coordinates 2303044..2303325 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5X95_RS11675 (2299409) 2299409..2300008 - 600 WP_015239195.1 rhomboid family intramembrane serine protease -
J5X95_RS11680 (2300101) 2300101..2300466 + 366 WP_094031521.1 holo-ACP synthase -
J5X95_RS11685 (2300631) 2300631..2301638 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
J5X95_RS11690 (2301755) 2301755..2302924 + 1170 WP_025284272.1 alanine racemase -
J5X95_RS11695 (2303044) 2303044..2303325 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
J5X95_RS11700 (2303331) 2303331..2303681 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
J5X95_RS11705 (2303799) 2303799..2304620 + 822 WP_003156182.1 STAS domain-containing protein -
J5X95_RS11710 (2304625) 2304625..2304990 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
J5X95_RS11715 (2304993) 2304993..2305394 + 402 WP_003156178.1 anti-sigma regulatory factor -
J5X95_RS11720 (2305406) 2305406..2306413 + 1008 WP_007410233.1 PP2C family protein-serine/threonine phosphatase -
J5X95_RS11725 (2306477) 2306477..2306806 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
J5X95_RS11730 (2306803) 2306803..2307285 + 483 WP_007609591.1 anti-sigma B factor RsbW -
J5X95_RS11735 (2307251) 2307251..2308039 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
J5X95_RS11740 (2308039) 2308039..2308641 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T197353 WP_003156187.1 NZ_CP072120:2303331-2303681 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T197353 NZ_CP096033:c1224116-1223370 [Bacillus amyloliquefaciens]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGAATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAAGCGGATAAGGACCGCTTGACAGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTTCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT197353 WP_003156188.1 NZ_CP072120:2303044-2303325 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT197353 NZ_CP096033:c1223369-1223199 [Bacillus amyloliquefaciens]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References