Detailed information of TA system TA197352

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 5505492..5506068
• Replicon: chromosome
• Accession: NZ_CP072119
• Organism: Klebsiella michiganensis strain 12084

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A1D8JNH2
• Locus tag: J5O09_RS25975
• Protein ID: WP_042933975.1
• Coordinates: 5505781..5506068 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: J5O09_RS25970
• Protein ID: WP_014837314.1
• Coordinates: 5505492..5505794 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5O09_RS25955 (J5O09_25950)	5502329..5502664	+	336	WP_032719937.1	endoribonuclease SymE	-
J5O09_RS25960 (J5O09_25955)	5503119..5504030	+	912	WP_119834584.1	acetamidase/formamidase family protein	-
J5O09_RS25965 (J5O09_25960)	5504027..5505370	+	1344	WP_119834583.1	APC family permease	-
J5O09_RS25970 (J5O09_25965)	5505492..5505794	-	303	WP_014837314.1	BrnA antitoxin family protein	Antitoxin
J5O09_RS25975 (J5O09_25970)	5505781..5506068	-	288	WP_042933975.1	BrnT family toxin	Toxin
J5O09_RS25980 (J5O09_25975)	5506318..5506761	-	444	WP_014837312.1	FosA family fosfomycin resistance glutathione transferase	-
J5O09_RS25985 (J5O09_25980)	5506755..5507663	-	909	WP_119834582.1	LysR family transcriptional regulator	-
J5O09_RS25990 (J5O09_25985)	5507751..5508533	+	783	WP_032693033.1	NAD(P)H-dependent oxidoreductase	-
J5O09_RS25995 (J5O09_25990)	5508681..5509265	+	585	WP_004098242.1	TetR/AcrR family transcriptional regulator	-
J5O09_RS26000 (J5O09_25995)	5509411..5510211	+	801	WP_014227752.1	winged helix-turn-helix domain-containing protein	-
J5O09_RS26005 (J5O09_26000)	5510208..5510726	+	519	WP_042944214.1	FidL-like protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (39-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11239.69 Da
• Isoelectric Point: 8.5899

>T197352 WP_042933975.1 NZ_CP072119:c5506068-5505781 [Klebsiella michiganensis]
MPMEFEWDANKARSNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGVIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T197352 NZ_CP096033:488783-489133 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11549.14 Da
• Isoelectric Point: 9.7962

>AT197352 WP_014837314.1 NZ_CP072119:c5505794-5505492 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSGIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT197352 NZ_CP096033:488496-488777 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A1D8JNH2