Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4819779..4820398 Replicon chromosome
Accession NZ_CP072119
Organism Klebsiella michiganensis strain 12084

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag J5O09_RS22820 Protein ID WP_004099646.1
Coordinates 4820180..4820398 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID H3N7X7
Locus tag J5O09_RS22815 Protein ID WP_004129911.1
Coordinates 4819779..4820153 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5O09_RS22805 (J5O09_22800) 4814936..4816129 + 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
J5O09_RS22810 (J5O09_22805) 4816152..4819298 + 3147 WP_014228207.1 multidrug efflux RND transporter permease subunit AcrB -
J5O09_RS22815 (J5O09_22810) 4819779..4820153 + 375 WP_004129911.1 Hha toxicity modulator TomB Antitoxin
J5O09_RS22820 (J5O09_22815) 4820180..4820398 + 219 WP_004099646.1 HHA domain-containing protein Toxin
J5O09_RS22825 (J5O09_22820) 4820561..4821127 + 567 WP_038424164.1 maltose O-acetyltransferase -
J5O09_RS29650 4821099..4821233 - 135 WP_223226764.1 hypothetical protein -
J5O09_RS22830 (J5O09_22825) 4821254..4821724 + 471 WP_014228205.1 YlaC family protein -
J5O09_RS22835 (J5O09_22830) 4821699..4823153 - 1455 WP_038424163.1 PLP-dependent aminotransferase family protein -
J5O09_RS22840 (J5O09_22835) 4823255..4823953 + 699 WP_014228203.1 GNAT family protein -
J5O09_RS22845 (J5O09_22840) 4823950..4824090 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
J5O09_RS22850 (J5O09_22845) 4824090..4824353 - 264 WP_004848323.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T197350 WP_004099646.1 NZ_CP072119:4820180-4820398 [Klebsiella michiganensis]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T197350 NZ_CP096018:4317494-4317757 [Alkalimarinus coralli]
ATGAGTAGCCGGTTACTTGTATGGACTGATGAAGCGTGGAGTGACTATTTGTATTGGCAAGGTCAGGATAAAAAGACGCT
AAAGCGTATTAACAAGCTTATCAGTGACTCCAAGAGAGCACCGTTCGAGGGGATAGGTAAACCAGAACCGTTAAAAGAGT
ACCTGTCTGGTTTTTGGTCTCGCAGAATTGATGATACAAATAGGTTGGTATATGCAGTAGATAATACATCACTAACAGTT
ATTTCTTGCAGATACCACTACTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14350.09 Da        Isoelectric Point: 4.8989

>AT197350 WP_004129911.1 NZ_CP072119:4819779-4820153 [Klebsiella michiganensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT197350 NZ_CP096018:4317247-4317501 [Alkalimarinus coralli]
ATGAAAATTGTATCTTTTACTGAAGCTCGCAATGGTTTAAAGGCTGTTTTGGATTCCGTTGTAAACGATGCAGATTCAGC
GATTATTACTCGCCGTGACTCTGAAGATGCTGTTGTAATGTCATTAAATTACTACAACAGTTTAATGGAAACTGTTCACT
TATTAAGATCCCCGGCTAATGCTGAGCATTTAGCCAAGTCTATTGATCAATTTAGAAAAGGGTCTGCCACTCCCAGAGAT
CTAATAGATGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HD25

References