Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2282504..2283094 Replicon chromosome
Accession NZ_CP072119
Organism Klebsiella michiganensis strain 12084

Toxin (Protein)


Gene name pemK Uniprot ID A0A0H3HDW1
Locus tag J5O09_RS10965 Protein ID WP_014229979.1
Coordinates 2282762..2283094 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID J6I3K7
Locus tag J5O09_RS10960 Protein ID WP_004852307.1
Coordinates 2282504..2282761 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5O09_RS10935 (J5O09_10935) 2277760..2278365 - 606 WP_032750440.1 glutathione S-transferase family protein -
J5O09_RS10940 (J5O09_10940) 2278545..2279471 + 927 WP_014229983.1 LysR substrate-binding domain-containing protein -
J5O09_RS10945 (J5O09_10945) 2279509..2280507 - 999 WP_014229982.1 aldo/keto reductase -
J5O09_RS10950 (J5O09_10950) 2280608..2281522 + 915 WP_014229981.1 LysR family transcriptional regulator -
J5O09_RS10955 (J5O09_10955) 2281713..2281793 - 81 Protein_2152 molybdopterin-binding protein -
J5O09_RS10960 (J5O09_10960) 2282504..2282761 + 258 WP_004852307.1 antitoxin Antitoxin
J5O09_RS10965 (J5O09_10965) 2282762..2283094 + 333 WP_014229979.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
J5O09_RS10975 (J5O09_10975) 2283417..2284874 + 1458 WP_014229916.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
J5O09_RS10985 (J5O09_10985) 2285837..2287291 - 1455 WP_014229909.1 AMP nucleosidase -
J5O09_RS10990 (J5O09_10990) 2287424..2287681 - 258 WP_014229908.1 histidine kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2272797..2283094 10297


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11740.57 Da        Isoelectric Point: 10.1863

>T197341 WP_014229979.1 NZ_CP072119:2282762-2283094 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGTKTTGVIRCDQPRTI
DMAARNGKRLESIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T197341 NZ_CP096018:c1200792-1200481 [Alkalimarinus coralli]
ATGGCTCAAATAATCTGGACTGAGCCCGCACTAAATGATTTGAACGATATTGCTGAGTATATTGCGGTCAGCAATCCGTA
TGCAGCTAAACAACTAGTAGAAAACGTTTTCAGCAATGTCCAGAGACTAGAACAATTTCCAGATTCAGGCAGAGTACCTG
ATGAGATTTCGAATCTAAATTACCGAGAAGTTGTAGTAAACCCTTGCCGTGTTTTTTACAAAATTGACATGGATTCGGTG
TATATCCTTCATGTGATGAGACAAGAAAGGGATTTAAGGAAGTTCTTACTGTCTACCACAAATGGAAGCTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9422.88 Da        Isoelectric Point: 4.4607

>AT197341 WP_004852307.1 NZ_CP072119:2282504-2282761 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT197341 NZ_CP096018:c1201037-1200780 [Alkalimarinus coralli]
ATGAGAACCGAAGTCGTTACGACATTAAAGAGGCAAGCAACGAAACTACTTGCAGACTTGCATGAGTCAAAAGAACCTAT
TCTCATAACTGAGCATGGTCAGCCATCAGCCTATTTGCTTGACGTTGCCGACTACGAATTGATGGTCAATCGGATGAAAA
TTCTAGAAGGTATTGCCAAGGGTGAGCAGGCTATTCGAGACGGCAGAACCATGGATATGGGTCAAGCAAAAGATAAAATG
AATAAATGGCTCAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3HDW1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HDW8

References