Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 57430..57977 Replicon chromosome
Accession NZ_CP072119
Organism Klebsiella michiganensis strain 12084

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0H3H5T3
Locus tag J5O09_RS00290 Protein ID WP_014227299.1
Coordinates 57430..57738 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag J5O09_RS00295 Protein ID WP_014227298.1
Coordinates 57741..57977 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5O09_RS00260 (J5O09_00260) 53077..53388 - 312 WP_014227304.1 PTS sugar transporter subunit IIB -
J5O09_RS00265 (J5O09_00265) 53683..54624 + 942 WP_014227303.1 LacI family DNA-binding transcriptional regulator -
J5O09_RS00270 (J5O09_00270) 54648..54941 - 294 WP_014227302.1 YicS family protein -
J5O09_RS00275 (J5O09_00275) 55151..56116 + 966 WP_038423881.1 Rpn family recombination-promoting nuclease/putative transposase -
J5O09_RS00280 (J5O09_00280) 56116..56373 + 258 WP_032718834.1 hypothetical protein -
J5O09_RS00285 (J5O09_00285) 56377..57279 - 903 WP_009654083.1 DMT family transporter -
J5O09_RS00290 (J5O09_00290) 57430..57738 - 309 WP_014227299.1 CcdB family protein Toxin
J5O09_RS00295 (J5O09_00295) 57741..57977 - 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
J5O09_RS00300 (J5O09_00300) 58083..59516 - 1434 WP_038423880.1 PTS N-acetylmuramic acid transporter subunit IIBC -
J5O09_RS00305 (J5O09_00305) 59541..60443 - 903 WP_038423879.1 N-acetylmuramic acid 6-phosphate etherase -
J5O09_RS00310 (J5O09_00310) 60604..61770 - 1167 WP_014839828.1 multidrug effflux MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11725.57 Da        Isoelectric Point: 7.2729

>T197336 WP_014227299.1 NZ_CP072119:c57738-57430 [Klebsiella michiganensis]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQREVVKASMDFLFDGI

Download         Length: 309 bp

>T197336 NZ_CP096017:4316932-4317186 [Alkalimarinus coralli]
ATGAAAATTGTATCTTTTACTGAAGCTCGCAATGGTTTAAAGGCTGTTTTGGATTCCGTTGTAAACGATGCAGATTCAGC
GATTATTACTCGCCGTGACTCTGAAGATGCTGTTGTAATGTCATTAAATTACTACAACAGTTTAATGGAAACTGTTCACT
TATTAAGATCCCCGGCTAATGCTGAGCATTTAGCCAAGTCTATTGATCAATTTAGAAAAGGGTCTGCCACTCCCAGAGAT
CTAATAGATGAGTAG

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT197336 WP_014227298.1 NZ_CP072119:c57977-57741 [Klebsiella michiganensis]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT197336 NZ_CP096017:4317179-4317442 [Alkalimarinus coralli]
ATGAGTAGCCGGTTACTTGTATGGACTGATGAAGCGTGGAGTGACTATTTGTATTGGCAAGGTCAGGATAAAAAGACGCT
AAAGCGTATTAACAAGCTTATCAGTGACTCCAAGAGAGCACCGTTCGAGGGGATAGGTAAACCAGAACCGTTAAAAGAGT
ACCTGTCTGGTTTTTGGTCTCGCAGAATTGATGATACAAATAGGTTGGTATATGCAGTAGATAATACATCACTAACAGTT
ATTTCTTGCAGATACCACTACTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H5T3

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References