Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 1002664..1003096 Replicon chromosome
Accession NZ_CP072118
Organism Clostridioides difficile isolate MAR286

Toxin (Protein)


Gene name zeta Uniprot ID R4ZD79
Locus tag J6L80_RS05015 Protein ID WP_003423072.1
Coordinates 1002938..1003096 (+) Length 53 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag J6L80_RS05010 Protein ID WP_000301765.1
Coordinates 1002664..1002936 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6L80_RS04970 (J6L80_04970) 998288..999199 + 912 WP_003423057.1 conjugal transfer protein -
J6L80_RS04975 (J6L80_04975) 999320..999697 + 378 WP_003423058.1 GntR family transcriptional regulator -
J6L80_RS04980 (J6L80_04980) 999698..1000561 + 864 WP_003423060.1 ABC transporter ATP-binding protein -
J6L80_RS04985 (J6L80_04985) 1000558..1001202 + 645 WP_003423062.1 ABC-2 transporter permease -
J6L80_RS04990 (J6L80_04990) 1001215..1001406 + 192 WP_003423063.1 hypothetical protein -
J6L80_RS04995 (J6L80_04995) 1001611..1001799 + 189 WP_009269672.1 cysteine-rich KTR domain-containing protein -
J6L80_RS05000 (J6L80_05000) 1001907..1002266 - 360 WP_003423065.1 helix-turn-helix transcriptional regulator -
J6L80_RS05005 (J6L80_05005) 1002432..1002647 + 216 WP_002321610.1 peptide-binding protein -
J6L80_RS05010 (J6L80_05010) 1002664..1002936 + 273 WP_000301765.1 antitoxin Antitoxin
J6L80_RS05015 (J6L80_05015) 1002938..1003096 + 159 WP_003423072.1 zeta toxin family protein Toxin
J6L80_RS05020 (J6L80_05020) 1003148..1003504 + 357 Protein_895 AAA family ATPase -
J6L80_RS05025 (J6L80_05025) 1003614..1004351 + 738 WP_002292226.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
J6L80_RS05030 (J6L80_05030) 1004521..1004781 + 261 Protein_897 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
J6L80_RS05035 (J6L80_05035) 1004884..1005762 + 879 WP_003423074.1 ParA family protein -
J6L80_RS05040 (J6L80_05040) 1006210..1006626 + 417 WP_003423075.1 sigma factor-like helix-turn-helix DNA-binding protein -
J6L80_RS05045 (J6L80_05045) 1006633..1006881 + 249 WP_002323370.1 helix-turn-helix domain-containing protein -
J6L80_RS19505 1007333..1007533 + 201 WP_021405844.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element erm(B) - 981662..1008997 27335


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(26-50)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 53 a.a.        Molecular weight: 5776.53 Da        Isoelectric Point: 10.3088

>T197327 WP_003423072.1 NZ_CP072118:1002938-1003096 [Clostridioides difficile]
MANIVNFTDKQFENRLNDNLEELVQGKKAVESPTAFLLGGQPGSGKTSLRRR

Download         Length: 159 bp

>T197327 NZ_CP096017:c858744-858400 [Alkalimarinus coralli]
ATGAAGGCAGTATTTATAGAGTTACCGCCATTTGAGCGGAATAGAGGGCACTATTTAGATGACAATGCTTACCGTAATTT
TCAAAATGAGATACTGAAAAATCCTACATCTGGTGATTTAATAAAAGGGACTTGCGGACTGCGAAAAGTGCGTTATAAGG
ATGAACGTAGACGAAAAGGAAAGCGTGGTGGTGTTAGGGTTATCTACTATTGGTGGAAAGAAGGATCACAGTTTTGGCTT
TTTACCATTTACGACAAAGATGAAGTTACAGACCTTACCAAAGCTGAGCTGAAGTTATTGGCAGACTTGTTGAAGCTTGA
AATTAAAGCGAGAAGCTTATTATGA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT197327 WP_000301765.1 NZ_CP072118:1002664-1002936 [Clostridioides difficile]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT197327 NZ_CP096017:c858403-858092 [Alkalimarinus coralli]
ATGAAACGTAATTTATTCGAAGAATTAAGTGAAGGGTTCCAGTCTTTAAAGGATGATAGAGAAGGGAAAACTACCCTAAA
ACAGCACTTGGTGATGGCGACAGAAACGCCTGAGGTGACTAGCGAGGAGCTAATCAGTTTAAGGGAAAGGTTGCACTTAT
CACAGGCTGTTTTTGCTGATTTTTTAAGAATCAATAAAAGAACATTAGAAAATTGGGAGCAAGGCCGAGTTAAACCAAAC
TCACAGGCAGCTCTGCTTATCAAAATGGTTGAAAAATACCCAGACACCATTCAACGGTTAGGTACTATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R4ZD79

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References