Detailed information of TA system TA197324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2799537..2800066
• Replicon: chromosome
• Accession: NZ_CP072116
• Organism: Staphylococcus aureus strain TCH32929

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: J6L63_RS13990
• Protein ID: WP_000621175.1
• Coordinates: 2799704..2800066 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: J6L63_RS13985
• Protein ID: WP_000948331.1
• Coordinates: 2799537..2799707 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6L63_RS13955 (2794574)	2794574..2795134	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
J6L63_RS13960 (2795342)	2795342..2795821	+	480	WP_001287087.1	hypothetical protein	-
J6L63_RS13965 (2795814)	2795814..2797397	+	1584	WP_001294626.1	PH domain-containing protein	-
J6L63_RS13970 (2797384)	2797384..2797875	+	492	WP_001205912.1	PH domain-containing protein	-
J6L63_RS13975 (2797879)	2797879..2798238	+	360	WP_000581197.1	holo-ACP synthase	-
J6L63_RS13980 (2798304)	2798304..2799452	+	1149	WP_001281154.1	alanine racemase	-
J6L63_RS13985 (2799537)	2799537..2799707	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
J6L63_RS13990 (2799704)	2799704..2800066	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
J6L63_RS13995 (2800416)	2800416..2801417	+	1002	WP_000390827.1	PP2C family protein-serine/threonine phosphatase	-
J6L63_RS14000 (2801536)	2801536..2801862	+	327	WP_001052491.1	anti-sigma factor antagonist	-
J6L63_RS14005 (2801864)	2801864..2802343	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
J6L63_RS14010 (2802318)	2802318..2803088	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T197324 WP_000621175.1 NZ_CP072116:2799704-2800066 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T197324 NZ_CP096016:c4344408-4344058 [Alkalimarinus coralli]
TTGGTAAGGCGTAAGCAGTTTGATCGAGGCGATATCGTGAAAGTCTCACTCAACCCCGTGGTAGGCCGTGAAATGCAAGG
CAATTTCAGGCCCTGTATGGTGTTGTCTCCAGCAGCGTTTAATCGCTTGGGTACGGCGTTGGTTGCCCCGATTACGCAAG
GCGGTGATTTCGCCAGAGTGAAGGGCTTTGCAGTTCCGCTAATTGGCTCCGGTACAGAGACTCAAGGCGTTATGCTGGTG
AATGCAGTACGAATGCTGGATCTGGAAGGCCGGCAAGCAAAAAAGGTTGAGGTCGCCCCCCAAGAGATCGTGGACGATGT
ATTGGCCCGACTTGCGGCCATCATCGAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT197324 WP_000948331.1 NZ_CP072116:2799537-2799707 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT197324 NZ_CP096016:c4344659-4344402 [Alkalimarinus coralli]
ATGAGAACTACAATTCGAAAGATTGGGAATAGCGCAGGTGCCATACTGCCAGCGACAGTCCTTAAGAAATTGAACTTGTC
AGAAGGCGATGTAATTGAGGTGTCGGAAGACGGCAAGCGTATTGTGATCGAGCCTCGCAATGAAAAGCCTAAATACAGCC
TGACAGAGCTTATGGCCCAGTGCGATGAAAAAGCCCCTCTACCCAAAGAACTGGAGGAATGGAATGACATGCAACCGACG
GGGAAAGAGGTTTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1