197291

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 5326946..5327588 Replicon chromosome
Accession NZ_CP072061
Organism Bacillus mycoides strain JAS06/1

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag J5V93_RS27355 Protein ID WP_000635965.1
Coordinates 5327238..5327588 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag J5V93_RS27350 Protein ID WP_000004570.1
Coordinates 5326946..5327233 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5V93_RS27325 (J5V93_27325) 5322261..5323223 + 963 WP_002009966.1 UV DNA damage repair endonuclease UvsE -
J5V93_RS27330 (J5V93_27330) 5323216..5323788 - 573 WP_002124818.1 rhomboid family intramembrane serine protease -
J5V93_RS27335 (J5V93_27335) 5323881..5324240 + 360 WP_002009969.1 holo-ACP synthase -
J5V93_RS27340 (J5V93_27340) 5324397..5325347 + 951 WP_002091373.1 outer membrane lipoprotein carrier protein LolA -
J5V93_RS27345 (J5V93_27345) 5325465..5326634 + 1170 WP_002124821.1 alanine racemase -
J5V93_RS27350 (J5V93_27350) 5326946..5327233 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
J5V93_RS27355 (J5V93_27355) 5327238..5327588 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
J5V93_RS27360 (J5V93_27360) 5327657..5329825 + 2169 WP_002029442.1 Tex family protein -
J5V93_RS27365 (J5V93_27365) 5329883..5329999 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
J5V93_RS27370 (J5V93_27370) 5330210..5330665 + 456 WP_016095131.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T197291 WP_000635965.1 NZ_CP072061:5327238-5327588 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T197291 NZ_CP095920:c141786-141505 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAAGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT197291 WP_000004570.1 NZ_CP072061:5326946-5327233 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT197291 NZ_CP095920:c142010-141783 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References