Detailed information of TA system TA197289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2403359..2404337
• Replicon: chromosome
• Accession: NZ_CP072057
• Organism: Bacillus mycoides strain JAS12/5

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: J5V73_RS12280
• Protein ID: WP_131246200.1
• Coordinates: 2403359..2404099 (+)
• Length: 247 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: R8HWP4
• Locus tag: J5V73_RS12285
• Protein ID: WP_000588712.1
• Coordinates: 2404212..2404337 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5V73_RS12250 (J5V73_12250)	2398460..2399221	+	762	WP_215577098.1	ABC transporter ATP-binding protein	-
J5V73_RS12255 (J5V73_12255)	2399328..2400038	+	711	WP_088294191.1	class I SAM-dependent methyltransferase	-
J5V73_RS12260 (J5V73_12260)	2400157..2400570	+	414	WP_002012925.1	VOC family protein	-
J5V73_RS12265 (J5V73_12265)	2400747..2401379	-	633	WP_002017047.1	type 1 glutamine amidotransferase family protein	-
J5V73_RS12270 (J5V73_12270)	2401653..2402129	+	477	WP_002017049.1	MarR family winged helix-turn-helix transcriptional regulator	-
J5V73_RS12275 (J5V73_12275)	2402283..2403020	+	738	WP_215577099.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
J5V73_RS12280 (J5V73_12280)	2403359..2404099	+	741	WP_131246200.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
J5V73_RS12285 (J5V73_12285)	2404212..2404337	+	126	WP_000588712.1	hypothetical protein	Antitoxin
J5V73_RS12290 (J5V73_12290)	2404413..2404589	+	177	WP_002017052.1	stage II sporulation protein SB	-
J5V73_RS12295 (J5V73_12295)	2404609..2404972	-	364	Protein_2365	YxeA family protein	-
J5V73_RS12300 (J5V73_12300)	2405244..2406713	+	1470	WP_131246201.1	aminoacyl-histidine dipeptidase	-
J5V73_RS12305 (J5V73_12305)	2407073..2407222	+	150	WP_002069316.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-244)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 247 a.a.
• Molecular weight: 28452.09 Da
• Isoelectric Point: 8.3034

>T197289 WP_131246200.1 NZ_CP072057:2403359-2404099 [Bacillus mycoides]
MTISNIRIGLFILAIVFIVLVFFYWRNEELYEEKKQRIRKTWYGLFITSVTVYFTIKGIDLTLWKNLLMFAAMVIFVDIA
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPTAFGTMEWHTEEEYTKSLNTFLDSYGEKIGAK
IVVFEAVKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIH
HSYFSK

Nucleotide

• Length: 741 bp

>T197289 NZ_CP095874:240336-240686 [Bacillus sp. PGP15]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CTAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4938.34 Da
• Isoelectric Point: 3.9150

>AT197289 WP_000588712.1 NZ_CP072057:2404212-2404337 [Bacillus mycoides]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Nucleotide

• Length: 126 bp

>AT197289 NZ_CP095874:240044-240331 [Bacillus sp. PGP15]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A366GQT1