Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 2370661..2371639 Replicon chromosome
Accession NZ_CP072055
Organism Bacillus mycoides strain JAS85/1

Toxin (Protein)


Gene name spoIISA Uniprot ID J8I6P2
Locus tag J5W00_RS12115 Protein ID WP_002017051.1
Coordinates 2370661..2371401 (+) Length 247 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID R8HWP4
Locus tag J5W00_RS12120 Protein ID WP_000588712.1
Coordinates 2371514..2371639 (+) Length 42 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5W00_RS12085 (J5W00_12085) 2365763..2366524 + 762 WP_002127420.1 ABC transporter ATP-binding protein -
J5W00_RS12090 (J5W00_12090) 2366630..2367340 + 711 WP_002127421.1 class I SAM-dependent methyltransferase -
J5W00_RS12095 (J5W00_12095) 2367459..2367872 + 414 WP_002012925.1 VOC family protein -
J5W00_RS12100 (J5W00_12100) 2368049..2368681 - 633 WP_002017047.1 type 1 glutamine amidotransferase family protein -
J5W00_RS12105 (J5W00_12105) 2368955..2369431 + 477 WP_002017049.1 MarR family winged helix-turn-helix transcriptional regulator -
J5W00_RS12110 (J5W00_12110) 2369585..2370322 + 738 WP_002032137.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
J5W00_RS12115 (J5W00_12115) 2370661..2371401 + 741 WP_002017051.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
J5W00_RS12120 (J5W00_12120) 2371514..2371639 + 126 WP_000588712.1 hypothetical protein Antitoxin
J5W00_RS12125 (J5W00_12125) 2371715..2371891 + 177 WP_003189253.1 stage II sporulation protein SB -
J5W00_RS12130 (J5W00_12130) 2371912..2372301 - 390 WP_033731043.1 YxeA family protein -
J5W00_RS12135 (J5W00_12135) 2372547..2374016 + 1470 WP_016126945.1 aminoacyl-histidine dipeptidase -
J5W00_RS12140 (J5W00_12140) 2374376..2374525 + 150 WP_002069316.1 hypothetical protein -
J5W00_RS12145 (J5W00_12145) 2375239..2375898 + 660 WP_003189261.1 CatB-related O-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(10-244)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 247 a.a.        Molecular weight: 28512.20 Da        Isoelectric Point: 8.3034

>T197287 WP_002017051.1 NZ_CP072055:2370661-2371401 [Bacillus mycoides]
MTISNIRIGLFILAIVFIVLVFFYWRNEELYEEKKQRIRKTWYGLFITSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIA
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPTAFGTMEWHTEEEYTKSLNTFLDSYGEKIGAK
IVVFEAVKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIH
HSYFSK

Download         Length: 741 bp

>T197287 NZ_CP095872:417813-418199 [Acinetobacter schindleri]
ATGGATAATCGTTGTTATCAGCTGAAACGCAGCCTGCTTGCACTTGCGTTTCAGTTTTTCATTTTTATTGTGTTGATGTT
TGTGATGTACCAGTTATTGCCCTTATGGCTATGGGGAATCTGTCTGGTTGCAGGACTGATCATCTATCATCTGTTTTATC
GCAAGCGTCCGCAGATCGCCCAGTTTGAATATTTGGATGGTCGGGAATGGACCATTACCCATCATGATCAGCGCATACAG
CGTGCCCAGATCAGTCATGTGATTGATCACCAAGCTTATCTGGTGGTGTATTTTCAGCATGCCAAAGTACCGCCATTGCT
GATCTGGTGTGACCAGTTACCAACACAACAATGGAAGTCATTGAAAGTCCTTACGAAAATGATCTGA

Antitoxin


Download         Length: 42 a.a.        Molecular weight: 4938.34 Da        Isoelectric Point: 3.9150

>AT197287 WP_000588712.1 NZ_CP072055:2371514-2371639 [Bacillus mycoides]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Download         Length: 126 bp

>AT197287 NZ_CP095872:417563-417820 [Acinetobacter schindleri]
ATGACTGAAGACATCAGCCTGGAAGAGCGTAAAGTGATTTACCGCGCACGTCGTGGTCTTAAAGAGTTGGATGTGTATTT
TGATCCCTATGTCAAAAACTGCTATTTGACAGCAGACCCATTTGAAAAAGAAATGTTTGCTGAACTCGTAGCGCAAGAAG
ACCCGGACCTGCTGGACTGGTTTATGGAAGTATCCGAACCGCCACGCCCTGAATTACGTGAGTTTATTAACAAGCTTAAA
CACTACGTTCATGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB J8I6P2

Antitoxin

Source ID Structure
AlphaFold DB A0A366GQT1

References