Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 245373..246015 Replicon chromosome
Accession NZ_CP072055
Organism Bacillus mycoides strain JAS85/1

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag J5W00_RS01380 Protein ID WP_000635965.1
Coordinates 245665..246015 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag J5W00_RS01375 Protein ID WP_000004570.1
Coordinates 245373..245660 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5W00_RS01350 (J5W00_01350) 240688..241650 + 963 WP_002009966.1 UV DNA damage repair endonuclease UvsE -
J5W00_RS01355 (J5W00_01355) 241643..242215 - 573 WP_002124818.1 rhomboid family intramembrane serine protease -
J5W00_RS01360 (J5W00_01360) 242308..242667 + 360 WP_002009969.1 holo-ACP synthase -
J5W00_RS01365 (J5W00_01365) 242824..243774 + 951 WP_002091373.1 outer membrane lipoprotein carrier protein LolA -
J5W00_RS01370 (J5W00_01370) 243892..245061 + 1170 WP_002124821.1 alanine racemase -
J5W00_RS01375 (J5W00_01375) 245373..245660 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
J5W00_RS01380 (J5W00_01380) 245665..246015 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
J5W00_RS01385 (J5W00_01385) 246084..248252 + 2169 WP_002029442.1 Tex family protein -
J5W00_RS01390 (J5W00_01390) 248310..248426 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
J5W00_RS01395 (J5W00_01395) 248637..249092 + 456 WP_215560863.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T197286 WP_000635965.1 NZ_CP072055:245665-246015 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T197286 NZ_CP095868:c54236-53949 [Acinetobacter nosocomialis]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT197286 WP_000004570.1 NZ_CP072055:245373-245660 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT197286 NZ_CP095868:c53962-53648 [Acinetobacter nosocomialis]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References