Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1966065..1966896 Replicon chromosome
Accession NZ_CP072054
Organism Escherichia coli strain K1508

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag J5N95_RS09680 Protein ID WP_000854814.1
Coordinates 1966522..1966896 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag J5N95_RS09675 Protein ID WP_001285584.1
Coordinates 1966065..1966433 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5N95_RS09660 (1963732) 1963732..1965264 + 1533 WP_001350525.1 protein YeeR -
J5N95_RS09665 (1965261) 1965261..1965707 + 447 WP_000187523.1 RadC family protein -
J5N95_RS09670 (1965770) 1965770..1965991 + 222 WP_000692323.1 DUF987 domain-containing protein -
J5N95_RS09675 (1966065) 1966065..1966433 + 369 WP_001285584.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
J5N95_RS09680 (1966522) 1966522..1966896 + 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
J5N95_RS09685 (1966893) 1966893..1967087 + 195 WP_000988600.1 DUF5983 family protein -
J5N95_RS09690 (1967133) 1967133..1967213 + 81 Protein_1908 hypothetical protein -
J5N95_RS09695 (1967502) 1967502..1967630 - 129 Protein_1909 transposase domain-containing protein -
J5N95_RS09700 (1967750) 1967750..1967884 + 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
J5N95_RS09705 (1967985) 1967985..1968314 - 330 WP_000450409.1 DUF496 family protein -
J5N95_RS09710 (1968486) 1968486..1969544 - 1059 WP_001200891.1 FUSC family protein -
J5N95_RS09715 (1969742) 1969742..1970215 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
J5N95_RS09720 (1970334) 1970334..1971500 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T197268 WP_000854814.1 NZ_CP072054:1966522-1966896 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T197268 NZ_CP095856:c2295114-2294800 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAAAACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT197268 WP_001285584.1 NZ_CP072054:1966065-1966433 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT197268 NZ_CP095856:c2294803-2294387 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References