Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1397815..1398453 Replicon chromosome
Accession NZ_CP072054
Organism Escherichia coli strain K1508

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag J5N95_RS06795 Protein ID WP_000813794.1
Coordinates 1397815..1397991 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag J5N95_RS06800 Protein ID WP_001270286.1
Coordinates 1398037..1398453 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5N95_RS06775 (1393434) 1393434..1394648 - 1215 WP_001326689.1 BenE family transporter YdcO -
J5N95_RS06780 (1394701) 1394701..1395237 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
J5N95_RS06785 (1395310) 1395310..1397271 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
J5N95_RS06790 (1397363) 1397363..1397593 - 231 WP_000494244.1 YncJ family protein -
J5N95_RS06795 (1397815) 1397815..1397991 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
J5N95_RS06800 (1398037) 1398037..1398453 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
J5N95_RS06805 (1398532) 1398532..1399938 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
J5N95_RS06810 (1400183) 1400183..1401328 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
J5N95_RS06815 (1401346) 1401346..1402359 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
J5N95_RS06820 (1402360) 1402360..1403301 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T197257 WP_000813794.1 NZ_CP072054:1397815-1397991 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T197257 NZ_CP095856:c331214-331107 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT197257 WP_001270286.1 NZ_CP072054:1398037-1398453 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT197257 NZ_CP095856:331261-331327 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References