Detailed information of TA system TA197246

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 382073..382691
• Replicon: chromosome
• Accession: NZ_CP072054
• Organism: Escherichia coli strain K1508

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: J5N95_RS01875
• Protein ID: WP_001290581.1
• Coordinates: 382073..382291 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: J5N95_RS01880
• Protein ID: WP_000344800.1
• Coordinates: 382317..382691 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5N95_RS01840 (377362)	377362..377934	+	573	WP_000779842.1	YbaY family lipoprotein	-
J5N95_RS01845 (377965)	377965..378276	-	312	WP_000409911.1	MGMT family protein	-
J5N95_RS01855 (378655)	378655..379008	+	354	WP_000878140.1	DUF1428 domain-containing protein	-
J5N95_RS01860 (379050)	379050..380600	-	1551	WP_001310610.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
J5N95_RS01865 (380764)	380764..381234	-	471	WP_000136192.1	YlaC family protein	-
J5N95_RS01870 (381350)	381350..381901	-	552	WP_000102564.1	maltose O-acetyltransferase	-
J5N95_RS01875 (382073)	382073..382291	-	219	WP_001290581.1	HHA domain-containing protein	Toxin
J5N95_RS01880 (382317)	382317..382691	-	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
J5N95_RS01885 (383237)	383237..386386	-	3150	WP_001132469.1	efflux RND transporter permease AcrB	-
J5N95_RS01890 (386409)	386409..387602	-	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8678.06 Da
• Isoelectric Point: 8.9008

>T197246 WP_001290581.1 NZ_CP072054:c382291-382073 [Escherichia coli]
MSEKFLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T197246 NZ_CP095843:3525776-3526066 [Escherichia fergusonii]
ATGAACTATGAACTGGCTTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGGGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTACGGGATTTGCCGGACTGCTACAAAATTA
AGCTTCGCACACTGGGATATCGCCGGGTCTATCAGGTCAATGATAAAGAGTTACTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACAAAACGGCTGGATGAGTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT197246 WP_000344800.1 NZ_CP072054:c382691-382317 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT197246 NZ_CP095843:3525544-3525786 [Escherichia fergusonii]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAGGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5