Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yafW-ykfI/CbtA-CbeA |
| Location | 262551..263230 | Replicon | chromosome |
| Accession | NZ_CP072054 | ||
| Organism | Escherichia coli strain K1508 | ||
Toxin (Protein)
| Gene name | ykfI | Uniprot ID | P77692 |
| Locus tag | J5N95_RS01240 | Protein ID | WP_000854672.1 |
| Coordinates | 262551..262892 (-) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | yafW | Uniprot ID | Q47684 |
| Locus tag | J5N95_RS01245 | Protein ID | WP_000070395.1 |
| Coordinates | 262913..263230 (-) | Length | 106 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| J5N95_RS01215 (257828) | 257828..258229 | + | 402 | WP_000174689.1 | sigma factor-binding protein Crl | - |
| J5N95_RS01220 (258268) | 258268..259323 | - | 1056 | WP_000749863.1 | phosphoporin PhoE | - |
| J5N95_RS01225 (259611) | 259611..260714 | + | 1104 | WP_001285288.1 | glutamate 5-kinase | - |
| J5N95_RS01230 (260726) | 260726..261979 | + | 1254 | WP_000893278.1 | glutamate-5-semialdehyde dehydrogenase | - |
| J5N95_RS01240 (262551) | 262551..262892 | - | 342 | WP_000854672.1 | type IV toxin-antitoxin system toxin YkfI | Toxin |
| J5N95_RS01245 (262913) | 262913..263230 | - | 318 | WP_000070395.1 | type IV toxin-antitoxin system antitoxin YafW | Antitoxin |
| J5N95_RS01250 (263249) | 263249..263470 | - | 222 | WP_000691994.1 | DUF987 domain-containing protein | - |
| J5N95_RS01255 (263479) | 263479..263955 | - | 477 | WP_000811693.1 | RadC family protein | - |
| J5N95_RS01260 (263971) | 263971..264429 | - | 459 | WP_000211838.1 | antirestriction protein | - |
| J5N95_RS01265 (264527) | 264527..264766 | - | 240 | WP_000194654.1 | DUF905 family protein | - |
| J5N95_RS01270 (264843) | 264843..265310 | - | 468 | WP_001547765.1 | protein YkfB | - |
| J5N95_RS01275 (265333) | 265333..265776 | - | 444 | WP_000824223.1 | lipoprotein YafY | - |
| J5N95_RS01280 (265776) | 265776..266003 | - | 228 | WP_001548158.1 | protein YpjK | - |
| J5N95_RS01285 (265999) | 265999..266190 | - | 192 | Protein_250 | DeoR family transcriptional regulator | - |
| J5N95_RS01290 (266407) | 266407..267228 | - | 822 | WP_000197389.1 | DUF932 domain-containing protein | - |
| J5N95_RS01295 (267320) | 267320..268183 | - | 864 | WP_001065553.1 | GTPase family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 12905.01 Da Isoelectric Point: 9.6543
>T197245 WP_000854672.1 NZ_CP072054:c262892-262551 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
Download Length: 342 bp
>T197245 NZ_CP095843:3262850-3263398 [Escherichia fergusonii]
ATGTCTCATAATTCAGATATCTATAAACTGATCGGAGCGGCCGCAGGTGTTGAAAATGGTCGCTCTGAAGCATCCTTAAA
CTCTACTGACAGTCAAGAGCAGGCTTTTGAATCCGCATTTGAGCCGTCTAATCATGAGCGCGTTGATGATTCTTCTTCAG
AAAATAAGGCTATTCTGGAGGCAGAAGAGTTTGGTTCTAATACTGGTGCATTGCATGAGTTTATGCAGCAGAACAGAATG
GACAGTCTTCAAGCTCAGCTTGATATGCTCAAGTCACAAGTACGGGATAAGATAGCTGACGCAACTGGTAAAGAAATAGA
CAATGAGCTTCGGACAAAAATGGCCTCATTCACGGTTTGGTTTATGTCGTGTTGGTGCTTATTTGTAGCCGGAATGTTTG
TATCATTTCTTATTGCTCATGAAGGAAAGCCGCCAGTTGAAGCAATCGTTGCATTACTGGGAACAAGTACAATTAGTATT
GTTGGTTTGGTTGGCTTCGTTGTTAGTGGATTGTTCAAATCTAGAAAAGATGGCGATAAAGAAAAATAA
ATGTCTCATAATTCAGATATCTATAAACTGATCGGAGCGGCCGCAGGTGTTGAAAATGGTCGCTCTGAAGCATCCTTAAA
CTCTACTGACAGTCAAGAGCAGGCTTTTGAATCCGCATTTGAGCCGTCTAATCATGAGCGCGTTGATGATTCTTCTTCAG
AAAATAAGGCTATTCTGGAGGCAGAAGAGTTTGGTTCTAATACTGGTGCATTGCATGAGTTTATGCAGCAGAACAGAATG
GACAGTCTTCAAGCTCAGCTTGATATGCTCAAGTCACAAGTACGGGATAAGATAGCTGACGCAACTGGTAAAGAAATAGA
CAATGAGCTTCGGACAAAAATGGCCTCATTCACGGTTTGGTTTATGTCGTGTTGGTGCTTATTTGTAGCCGGAATGTTTG
TATCATTTCTTATTGCTCATGAAGGAAAGCCGCCAGTTGAAGCAATCGTTGCATTACTGGGAACAAGTACAATTAGTATT
GTTGGTTTGGTTGGCTTCGTTGTTAGTGGATTGTTCAAATCTAGAAAAGATGGCGATAAAGAAAAATAA
Antitoxin
Download Length: 106 a.a. Molecular weight: 11939.59 Da Isoelectric Point: 6.7391
>AT197245 WP_000070395.1 NZ_CP072054:c263230-262913 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
Download Length: 318 bp
>AT197245 NZ_CP095843:3262354-3262827 [Escherichia fergusonii]
ATGTACAGTCCGGTACAAATAGCCAATAAATTCATAACGTTGGGAAACCAACATCATAATCCTCTGACCCACATGCAACT
GCAGAAGCTTACTTATATTGCCCATGGTTACTATTTAGCATTAACAGGTAAGCCTTTGCTCAATGAATGTGTCTCCGCGT
GGAAGTACGGTCCAGTTATTCCTGGAATGTACGATGCTTTCAAAGACTATGGGAATAAACCTGTTACGAATGTAGCAGTA
GCTCCTTTTGGTGGCATCGTTACTATGGATCCGCAAGCAGAGAGCATTATAGGGGCTGTTTATAAGTTTTACGGCTCGAA
AAATGGAATTGAGTTATCAACTCTAACTCATATGCCAGGTACGCCTTGGTCACAAGCTTATAATGGTATTGGCTCGTCGA
TCATCTCAAATGATGCGATCAAGGCTTATTATCATGATTTATTGAATAACCGACAGCAATGTCAGGGCCTCTGA
ATGTACAGTCCGGTACAAATAGCCAATAAATTCATAACGTTGGGAAACCAACATCATAATCCTCTGACCCACATGCAACT
GCAGAAGCTTACTTATATTGCCCATGGTTACTATTTAGCATTAACAGGTAAGCCTTTGCTCAATGAATGTGTCTCCGCGT
GGAAGTACGGTCCAGTTATTCCTGGAATGTACGATGCTTTCAAAGACTATGGGAATAAACCTGTTACGAATGTAGCAGTA
GCTCCTTTTGGTGGCATCGTTACTATGGATCCGCAAGCAGAGAGCATTATAGGGGCTGTTTATAAGTTTTACGGCTCGAA
AAATGGAATTGAGTTATCAACTCTAACTCATATGCCAGGTACGCCTTGGTCACAAGCTTATAATGGTATTGGCTCGTCGA
TCATCTCAAATGATGCGATCAAGGCTTATTATCATGATTTATTGAATAACCGACAGCAATGTCAGGGCCTCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|