Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 5518467..5520196 Replicon chromosome
Accession NZ_CP072011
Organism Pseudomonas corrugata strain 3prime

Toxin (Protein)


Gene name pmenT Uniprot ID A0A8B6UP12
Locus tag C4C32_RS24325 Protein ID WP_055137583.1
Coordinates 5518467..5519111 (+) Length 215 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID A0A8B6UP13
Locus tag C4C32_RS24330 Protein ID WP_055137582.1
Coordinates 5519108..5520196 (+) Length 363 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4C32_RS24300 (C4C32_24300) 5514009..5514920 + 912 WP_024780091.1 restriction endonuclease -
C4C32_RS24305 (C4C32_24305) 5515014..5515568 + 555 WP_208554993.1 hypothetical protein -
C4C32_RS24310 (C4C32_24310) 5515951..5516310 - 360 WP_053191369.1 DUF6124 family protein -
C4C32_RS24315 (C4C32_24315) 5516806..5518056 + 1251 WP_024781172.1 IS256 family transposase -
C4C32_RS24320 (C4C32_24320) 5518137..5518466 + 330 WP_244251413.1 DarT ssDNA thymidine ADP-ribosyltransferase family protein -
C4C32_RS24325 (C4C32_24325) 5518467..5519111 + 645 WP_055137583.1 DUF4433 domain-containing protein Toxin
C4C32_RS24330 (C4C32_24330) 5519108..5520196 + 1089 WP_055137582.1 macro domain-containing protein Antitoxin
C4C32_RS24335 (C4C32_24335) 5520388..5520747 - 360 WP_055137581.1 DUF6124 family protein -
C4C32_RS24340 (C4C32_24340) 5521230..5521688 - 459 WP_055137580.1 GNAT family N-acetyltransferase -
C4C32_RS24345 (C4C32_24345) 5521975..5522190 + 216 WP_024780087.1 DUF1737 domain-containing protein -
C4C32_RS24350 (C4C32_24350) 5522563..5523459 + 897 WP_024780086.1 HTH-type transcriptional regulator ArgP -
C4C32_RS24355 (C4C32_24355) 5523634..5524116 + 483 WP_033034809.1 hypothetical protein -
C4C32_RS24360 (C4C32_24360) 5524091..5524606 + 516 WP_024780084.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 5515951..5524606 8655
- flank IS/Tn - - 5516806..5518056 1250


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(14-213)

Antitoxin

Macro domain

(18-130)


Sequences

Toxin        


Download         Length: 215 a.a.        Molecular weight: 24929.51 Da        Isoelectric Point: 8.4285

>T197238 WP_055137583.1 NZ_CP072011:5518467-5519111 [Pseudomonas corrugata]
MIYSRLNPEKALIWRIVHRDNLPWILDNGLHCANSTVRSSQYVNIGNADLIDKRRTRHVPIAPGGVLSDYVPFYFTPFSV
MMKNIHSGRSVQQRSNDEILILVSSLYLVEQMGFPFVYTNAHAYPDWTDYYSDLANLDQIDWSIIQRRDFKREPDDPRKM
ERYQAEALIHNHLPITGLLGIMCYTDAMKKRIDRDVAARGLTLSVHARPGWYFQ

Download         Length: 645 bp

>T197238 NZ_CP095843:c1766735-1766358 [Escherichia fergusonii]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGACCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCCGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCTGGAATTC
AGTGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGAGCTCGCCGGGCGACTGGCCTGATGACCCGCGA
CAATTACCGAACGGTAAATAACATTACCCTGGGTAAGTATCAGGAGGCGAAACGATGA

Antitoxin


Download         Length: 363 a.a.        Molecular weight: 40827.81 Da        Isoelectric Point: 6.4007

>AT197238 WP_055137582.1 NZ_CP072011:5519108-5520196 [Pseudomonas corrugata]
MIRFTQGNLLEAKTEALVNTVNTVGVMGKGIALMFKERFAENYRLYSAACKAREVETGKVHVTAVNELEGPRWIVNFPTK
RHWRSPSQMAWITEGLHDLRRFLIENDVKSVAVPPLGAGNGGLKWPEVREQIVEILSDLDVDVLVFEPSNQYLNISKRSG
VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERAIEQLPNTENPLNLRFVAHKYGPYANRLEHLLNNLDGSYLH
CDKRISDAGISDVIWFDEGRKAFLQAYLQTEAKEYTRALERTAELIDGFESPFGMELLATVDWLLSQDGISPTVPAVREA
LKHWDGGAGAAARKSKLFDDQALDIALKRLTSSSFRPEVATC

Download         Length: 1089 bp

>AT197238 NZ_CP095843:c1767192-1766824 [Escherichia fergusonii]
GTGTCAGACACGTTCCATGAAACAAATTATCCCGACAACAACAACGATCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCACTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B6UP12

Antitoxin

Source ID Structure
AlphaFold DB A0A8B6UP13

References