Detailed information of TA system TA197230

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1014387..1015192
• Replicon: chromosome
• Accession: NZ_CP071992
• Organism: Staphylococcus epidermidis strain CBPA-ST-11003

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: -
• Locus tag: J4Z27_RS04840
• Protein ID: WP_002457885.1
• Coordinates: 1015010..1015192 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: A0A0E1VBD1
• Locus tag: J4Z27_RS04835
• Protein ID: WP_002446763.1
• Coordinates: 1014387..1014986 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4Z27_RS04815 (J4Z27_000959)	1010097..1011554	+	1458	WP_002446767.1	ABC transporter substrate-binding protein/permease	-
J4Z27_RS04820 (J4Z27_000960)	1011547..1012269	+	723	WP_002446766.1	amino acid ABC transporter ATP-binding protein	-
J4Z27_RS04825 (J4Z27_000961)	1012632..1013762	+	1131	WP_002476052.1	tRNA epoxyqueuosine(34) reductase QueG	-
J4Z27_RS04830 (J4Z27_000962)	1013759..1014229	+	471	WP_002446764.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
J4Z27_RS04835 (J4Z27_000963)	1014387..1014986	+	600	WP_002446763.1	glucosamine-6-phosphate isomerase	Antitoxin
J4Z27_RS04840 (J4Z27_000964)	1015010..1015192	+	183	WP_002457885.1	SAS053 family protein	Toxin
J4Z27_RS04845 (J4Z27_000965)	1015344..1015748	+	405	WP_002446761.1	hypothetical protein	-
J4Z27_RS04850 (J4Z27_000966)	1015940..1017325	+	1386	WP_002447846.1	class II fumarate hydratase	-
J4Z27_RS04855 (J4Z27_000967)	1017800..1018282	+	483	Protein_924	transposase	-
J4Z27_RS04860 (J4Z27_000968)	1018456..1019280	-	825	WP_119557732.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1017971..1018282	311

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (125-195)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 7080.67 Da
• Isoelectric Point: 4.3016

>T197230 WP_002457885.1 NZ_CP071992:1015010-1015192 [Staphylococcus epidermidis]
MANKKDSKLNYHEEENAMVTDLDDLKELGKEMEQISQENDEEKLNQPHDNEVRSDFKNSK

Nucleotide

• Length: 183 bp

>T197230 NZ_CP095792:c51054-50773 [Escherichia fergusonii]
ATGATTTATGAACTGGCATTTGACCACAGAGCACTGAAGGAATGGCAAAAACTCGGTCACACTGTTCGTGAGCAATTCAA
AAAGAAACTGTCAGAACGCCTGATAAATCCACGCGTCCCCGCAGCCAAGCTACATGGTCATACTGACCGTTACAAAATTA
AGCTTCGCGCATCCGGTTACAGGCTCGTTTATCAGGTAATTGATGAGAAGGTCGTTTTGCTTGTGATTTCTGTGGGAAAA
AGAGAAGGCAGCGACGTCTATCACATGGCAGATACCCGCTGA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22635.54 Da
• Isoelectric Point: 4.9942

>AT197230 WP_002446763.1 NZ_CP071992:1014387-1014986 [Staphylococcus epidermidis]
MAMNFKVFDNSEKVAEYTADILRKQFNNNPTTIAGFHLGKEHAPVFDELKKNVENHAVDFSQINILDYDDNHSYYEALGV
PTGQIYSISYENDAIDFISDKIKTKENKGKLTMQVLTIDENGKLDVSVRQGLMEAREIFLVITGANKRDMVEKLYRENGK
TSFEPSDLKAHRMVNVILDKEAAAGLPEDVKEYFTARFA

Nucleotide

• Length: 600 bp

>AT197230 NZ_CP095792:c51295-51044 [Escherichia fergusonii]
ATGAGCTACCAAATTCTGACTACGACAGCAGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACTGTAGCCGAAGG
AGAAGGGGATGCCGTCGCAATCCTGAACCGAAATGAACCAGCGTTCTACTGTGTCCCACCAGAACTTTATGCCTACTACC
GGGAACTCGCTGAAGACGCTGAACTCAACGCAATTGCTGATGAAAGAATGAAAAATCCAGACATTGTAAGGGTTAACCTG
GATGATTTATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E1VBD1