Detailed information of TA system TA197227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 1947825..1948295
• Replicon: chromosome
• Accession: NZ_CP071988
• Organism: Staphylococcus epidermidis strain CBPA-ST-10002 isolate HPB10235

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: Q5HQS5
• Locus tag: J4Z26_RS09170
• Protein ID: WP_001829569.1
• Coordinates: 1948107..1948295 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: D4FNP2
• Locus tag: J4Z26_RS09165
• Protein ID: WP_002438963.1
• Coordinates: 1947825..1948082 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4Z26_RS09125 (J4Z26_001818)	1943191..1943907	-	717	WP_001831974.1	type I 3-dehydroquinate dehydratase	-
J4Z26_RS09130 (J4Z26_001819)	1944068..1944490	+	423	WP_002457221.1	organic hydroperoxide resistance protein	-
J4Z26_RS09135 (J4Z26_001820)	1944674..1944811	-	138	WP_162161215.1	hypothetical protein	-
J4Z26_RS09140	1944841..1945028	-	188	Protein_1771	hypothetical protein	-
J4Z26_RS09145 (J4Z26_001821)	1945415..1945915	+	501	WP_001829623.1	GNAT family N-acetyltransferase	-
J4Z26_RS09150 (J4Z26_001822)	1946186..1946773	-	588	WP_001829615.1	histidine phosphatase family protein	-
J4Z26_RS09155 (J4Z26_001823)	1946985..1947221	+	237	WP_002457224.1	hypothetical protein	-
J4Z26_RS09160 (J4Z26_001824)	1947213..1947416	-	204	WP_002438966.1	sterile alpha motif-like domain-containing protein	-
J4Z26_RS09165	1947825..1948082	+	258	WP_002438963.1	hypothetical protein	Antitoxin
J4Z26_RS09170 (J4Z26_001825)	1948107..1948295	+	189	WP_001829569.1	hypothetical protein	Toxin
J4Z26_RS09175 (J4Z26_001826)	1948485..1949038	+	554	Protein_1778	hypothetical protein	-
J4Z26_RS09180 (J4Z26_001827)	1949098..1949331	-	234	WP_001829664.1	hypothetical protein	-
J4Z26_RS09185 (J4Z26_001828)	1949615..1950361	+	747	WP_002438959.1	poly-gamma-glutamate hydrolase family protein	-
J4Z26_RS09190 (J4Z26_001829)	1950460..1950744	+	285	WP_001829635.1	hypothetical protein	-
J4Z26_RS09195 (J4Z26_001830)	1950768..1950992	+	225	WP_001829613.1	hypothetical protein	-
J4Z26_RS09200 (J4Z26_001831)	1951613..1951813	-	201	WP_001829681.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1944674..1944829	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7229.94 Da
• Isoelectric Point: 9.7844

>T197227 WP_001829569.1 NZ_CP071988:1948107-1948295 [Staphylococcus epidermidis]
MSLHFQILLWLSILFIIAGTILLVTMLKTKKEERKESYLGFTVIFLIFGFAILIYTFIFGIL

Nucleotide

• Length: 189 bp

>T197227 NZ_CP095788:113011-113316 [Escherichia fergusonii]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGTGATGAAAGCTGGCGCATGATGACCTCCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGGGAAAATGACATCAAAAACGCCATTAACCTGATGTTTTGGGGAATATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 10014.05 Da
• Isoelectric Point: 4.5440

>AT197227 WP_002438963.1 NZ_CP071988:1947825-1948082 [Staphylococcus epidermidis]
MKLNKANIFNLIFTILFFSFNILITYNANIDYKLWLIPGLAICGFALFSSLTLVIIYSDLFSEILFFINIILALYYIYPI
FYEFV

Nucleotide

• Length: 258 bp

>AT197227 NZ_CP095788:112791-113009 [Escherichia fergusonii]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0N0LUH8

Antitoxin

• AlphaFold DB: A0A0U5IVM3