Detailed information of TA system TA197199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 210096..210713
• Replicon: plasmid p1VB13828
• Accession: NZ_CP071959
• Organism: Enterococcus faecium strain VB13828

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: J4767_RS14370
• Protein ID: WP_224451579.1
• Coordinates: 210561..210713 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A829EWE8
• Locus tag: J4767_RS14365
• Protein ID: WP_002305052.1
• Coordinates: 210096..210527 (-)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4767_RS14350 (J4767_14350)	205911..207338	-	1428	WP_002361237.1	glycoside hydrolase family 32 protein	-
J4767_RS14355 (J4767_14355)	207392..208666	-	1275	WP_002323715.1	MFS transporter	-
J4767_RS14360 (J4767_14360)	208969..209922	+	954	WP_002325884.1	IS30 family transposase	-
J4767_RS14365 (J4767_14365)	210096..210527	-	432	WP_002305052.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
J4767_RS14370 (J4767_14370)	210561..210713	-	153	WP_224451579.1	type II toxin-antitoxin system HicA family toxin	Toxin
J4767_RS14375 (J4767_14375)	210807..210986	-	180	Protein_238	hypothetical protein	-
J4767_RS15620	211002..211202	-	201	WP_002324500.1	hypothetical protein	-
J4767_RS14380 (J4767_14380)	211367..212062	-	696	WP_002305050.1	hypothetical protein	-
J4767_RS14385 (J4767_14385)	212055..212492	-	438	WP_002305049.1	hypothetical protein	-
J4767_RS14390 (J4767_14390)	212508..212759	-	252	WP_002287517.1	hypothetical protein	-
J4767_RS14395 (J4767_14395)	212759..212965	-	207	WP_002324499.1	hypothetical protein	-
J4767_RS14400 (J4767_14400)	213413..213637	-	225	WP_074399798.1	hypothetical protein	-
J4767_RS14405 (J4767_14405)	213805..213949	+	145	Protein_245	type I toxin-antitoxin system toxin PepG1	-
J4767_RS14410 (J4767_14410)	214108..214365	-	258	WP_002295625.1	hypothetical protein	-
J4767_RS14415 (J4767_14415)	214387..214872	-	486	Protein_247	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(L) / ant(6)-Ia / aph(3')-III / erm(B) / aac(6')-aph(2'')	-	1..296092	296092
	-	flank	IS/Tn	-	-	208969..209922	953

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (2-49)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (10-104)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5815.65 Da
• Isoelectric Point: 10.3560

>T197199 WP_224451579.1 NZ_CP071959:c210713-210561 [Enterococcus faecium]
IEKNGWVERRQEGFHHHLYKDGVRITVPVHANQDLGRGLERKILKDAGLK

Nucleotide

• Length: 153 bp

>T197199 NZ_CP095776:352710-353057 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15811.92 Da
• Isoelectric Point: 4.1638

>AT197199 WP_002305052.1 NZ_CP071959:c210527-210096 [Enterococcus faecium]
MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYGIAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDD
SFTTLIRVDVAKYLKDTELVKKTLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN

Nucleotide

• Length: 432 bp

>AT197199 NZ_CP095776:353057-353356 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829EWE8