Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 18323..19460 Replicon plasmid p1VB13828
Accession NZ_CP071959
Organism Enterococcus faecium strain VB13828

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag J4767_RS13305 Protein ID WP_001284311.1
Coordinates 18323..19186 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag J4767_RS13310 Protein ID WP_000301765.1
Coordinates 19188..19460 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J4767_RS13275 (J4767_13275) 13895..15190 - 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
J4767_RS13280 (J4767_13280) 15435..15686 + 252 Protein_17 GNAT family N-acetyltransferase -
J4767_RS13285 (J4767_13285) 15779..16573 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
J4767_RS15720 (J4767_13290) 16983..17066 + 84 Protein_19 MLS leader peptide -
J4767_RS13295 (J4767_13295) 17115..17198 + 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
J4767_RS13300 (J4767_13300) 17323..18060 + 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
J4767_RS13305 (J4767_13305) 18323..19186 - 864 WP_001284311.1 zeta toxin family protein Toxin
J4767_RS13310 (J4767_13310) 19188..19460 - 273 WP_000301765.1 antitoxin Antitoxin
J4767_RS13315 (J4767_13315) 19477..19692 - 216 WP_001835296.1 peptide-binding protein -
J4767_RS13320 (J4767_13320) 19784..20035 - 252 Protein_25 chromosome partitioning protein ParA -
J4767_RS13325 (J4767_13325) 20090..20770 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
J4767_RS13330 (J4767_13330) 20848..21051 - 204 Protein_27 hypothetical protein -
J4767_RS13335 (J4767_13335) 21129..21671 - 543 WP_002302857.1 hypothetical protein -
J4767_RS13340 (J4767_13340) 21732..24051 - 2320 Protein_29 hypothetical protein -
J4767_RS13345 (J4767_13345) 24048..24413 - 366 Protein_30 type IV secretory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(L) / ant(6)-Ia / aph(3')-III / erm(B) / aac(6')-aph(2'') - 1..296092 296092
- inside IScluster/Tn tet(L) / ant(6)-Ia / aph(3')-III / erm(B) - 6177..25146 18969


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T197197 WP_001284311.1 NZ_CP071959:c19186-18323 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T197197 NZ_CP095775:c29759-29382 [Pseudomonas aeruginosa]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGTGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT197197 WP_000301765.1 NZ_CP071959:c19460-19188 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT197197 NZ_CP095775:c29401-29066 [Pseudomonas aeruginosa]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References